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APOA2 抗体

This 山羊 多克隆 antibody specifically detects APOA2 in WB, IP, IHC (p), EIA 和 IHC (fro). It exhibits reactivity toward 人.
产品编号 ABIN116542
发货至: 中国
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Quick Overview for APOA2 抗体 (ABIN116542)

抗原

See all APOA2 抗体
APOA2 (Apolipoprotein A-II (APOA2))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This APOA2 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • 特异性

    This product has been prepared by Immunoaffinity Chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1 % cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with ApoLipoprotein A-II and has negligible cross-reactivity with Type A-I, B, C-I, C-II, C-III, E and J apoLipoproteins. Non-specific cross reaction of anti-apoLipoprotein antibodies with other Human serum proteins is negligible.

    纯化方法

    Immunoaffinity Chromatography.

    免疫原

    ApoLipoprotein Type A-II was isolated from Human plasma by Density Gradient Centrifugation followed by HPLC purification.
  • 应用备注

    This Apo AII antibody R1031P has been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for Immunoprecipitation and Western blotting forhighly sensitive qualitative analysis. Recommended Dilutions: ELISA: 1/5,000-1/10,000. Immunoprecipitation: 1/100. Western blot: 1/5,000-1/10,000. Immunohistochemistry: 1: /50-1/200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL (by UV absorbance at 280 nm)

    缓冲液

    0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 and 0.01 % Sodium Azide as preservative.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    APOA2 (Apolipoprotein A-II (APOA2))

    别名

    Apolipoprotein A II (Apo AII)

    背景

    Apolipoprotein A II is the second most abundant protein of the high density lipoprotein particles. The apolipoprotein A II gene consists of 4 exons and 3 introns. The four exons encode the 5' untranslated region, pre peptide, a short N terminal domain and a C terminal domain composed of a variable number of lipid binding amphipathic helices. Familial apolipoprotein A II deficiency may result from a splice junction alteration which blocks splicing of intron 3 from the primary transcript and result in the formation of a non functional mRNA.Synonyms: APOA2, Apo-AII, ApoA-II, Apolipoprotein A-II, Apolipoprotein A2

    基因ID

    336

    NCBI登录号

    NP_001634

    UniProt

    P02652

    途径

    Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Negative Regulation of Transporter Activity, Lipid Metabolism
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