NFYB 抗体 (N-Term)
Quick Overview for NFYB 抗体 (N-Term) (ABIN116538)
抗原
See all NFYB 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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序列
- QQI SGV QQI QFS.
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特异性
- This product was prepared from monospecific antiserum by a multi-step procedure which includes delipidation, salt fractionation and ion exchange chromatography. A single precipitin arc was observed against anti-Rabbit Serum when assayed by immunoelectrophoresis. Reacts with Human and Mouse. Does not recognise the A subunit.
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纯化方法
- Prepared from monospecific antiserum by delipidation, salt fractionation
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免疫原
- NF-Y (B subunit specific) peptide corresponding to a region near the N-terminus of the Human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
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anti-Nuclear Transcription Factor Y, beta (NFYB) (AA 1-207) antibody
NFYB 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 5D1 unconjugated
anti-Nuclear Transcription Factor Y, beta (NFYB) (AA 1-207) antibodyNFYB 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 6H6 unconjugated
anti-Nuclear Transcription Factor Y, beta (NFYB) (AA 1-207) antibodyNFYB 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Transcription Factor Y, beta (NFYB) (AA 1-207) antibodyNFYB 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1G10 unconjugated
anti-Nuclear Transcription Factor Y, beta (NFYB) (N-Term) antibodyNFYB 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Transcription Factor Y, beta (NFYB) (N-Term) antibodyNFYB 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Transcription Factor Y, beta (NFYB) antibodyNFYB 适用: 人, 小鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Transcription Factor Y, beta (NFYB) (N-Term) antibodyNFYB 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Transcription Factor Y, beta (NFYB) (C-Term) antibodyNFYB 适用: 人, 小鼠, 大鼠, 犬, Pig, 豚鼠, 兔, 小鸡, 猴, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Transcription Factor Y, beta (NFYB) (N-Term) antibodyNFYB 适用: 人, 小鼠, 大鼠, 犬, Cow, Pig, 豚鼠, 兔, 马, 小鸡, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Suitable for Immunoprecipitation, Western blotting (1/500), ELISA (1/4,000) and supershiftassays. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against the 25 kDa B subunit of NF-Y at a dilution of 1: 500 followed by reactionwith Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat). Anti-NF-Y (Bsubunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y (Bsubunit specific). Minimal reaction was observed by immunoblot against the 35 kDa Asubunit of NF-Y. This product was also tested in a gel supershift assay and found to bereactive against human and mouse NF-Y using 2.0 to 4.0 l per assay. This product was assayed against NF-Y B subunit peptide in an antibody capture ELISAusing Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTSsubstrate. A dilution of 1: 4000 is suggested from this experiment. Minimal reactivity was
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.01 M Sodium Phosphate, 0.11 M Sodium Chloride, pH 7.2 with 0.01 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. Dilute only to immedoate use.
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储存条件
- -20 °C
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储存方法
- Store original vial at -20 °C or below. After opening aliquot contents and freeze at -20 °C or below.
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- NFYB (Nuclear Transcription Factor Y, beta (NFYB))
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别名
- NFYB
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背景
- The Y box is a CCAAT box which is bound by the heteromeric DNA binding protein, NF-Y (also known as CBF and CP1). Unlike the transcription factors C/EBP and CTF/NF1 which also bind CCAAT-like sequences, NF-Y exhibits a strict binding requirement for this pentanucleotide sequence. Binding sites for this factor have been described for nearly 30 % of all eukaryotic genes. Y/CCAAT sequences were frequently observed in the promoter-proximal sequences. NF-Y is composed of 3 separate subunits (A,B and C) each of which is required for DNA binding. Each subunit has remained highly conserved throughout evolution. In fact, homologous yeast subunits can substitute for mammalian NF-Y in DNA-binding assays. The conserved core sequences of NF-YB and NF-YC contain a 70 aa region similar to the histone fold motif of nucleosomes H2A and H2B. The unique structure and evolutionary conservation of this transcription factor suggests that it plays a fundamental role in the readout of eukaryotic genetic information.Synonyms: HAP3, Nuclear transcription factor Y subunit beta
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基因ID
- 4801
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NCBI登录号
- NP_006157
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UniProt
- P25208
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途径
- Regulation of Lipid Metabolism by PPARalpha
抗原
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