NFYA 抗体 (N-Term)
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- 抗原 See all NFYA 抗体
- NFYA (Nuclear Transcription Factor Y, alpha (NFYA))
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抗原表位
- N-Term
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This NFYA antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA)
- 特异性
- This product was prepared from monospecific antiserum by a multi-step procedure that includes delipidation, salt fractionation and ion exchange chromatography. A single precipitin arc was observed against anti-Rabbit Serum when assayed by Immunoelectrophoresis. No reactivity was observed by ELISA against the B subunit of NFY.
- 纯化方法
- Multistep process.
- 免疫原
- NF-Y (A subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
- Top Product
- Discover our top product NFYA Primary Antibody
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anti-Nuclear Transcription Factor Y, alpha (NFYA) (C-Term) antibody
NFYA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Transcription Factor Y, alpha (NFYA) (C-Term) antibodyNFYA 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, Pig, Bat, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Transcription Factor Y, alpha (NFYA) (N-Term) antibodyNFYA 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Transcription Factor Y, alpha (NFYA) (C-Term) antibodyNFYA 适用: 人, 小鼠, 大鼠, 犬, Cow, Pig WB, IHC, IF, IP, IC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Transcription Factor Y, alpha (NFYA) (C-Term) antibodyNFYA 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Transcription Factor Y, alpha (NFYA) (C-Term) antibodyNFYA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Transcription Factor Y, alpha (NFYA) (C-Term) antibodyNFYA 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, Saccharomyces cerevisiae WB, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Suitable for Immunoprecipitation, Immunoblotting, ELISA and supershift assays. Recommended Dilution(s): This product was assayed by Immunoblot and found to be reactive against thedifferentially spliced 35 and 42 kDa forms of the A subunit of human and mouse NF-Y at adilution of 1: 1000 followed by reaction with Peroxidase conjugated Affinity Purifiedanti-Rabbit IgG [H&L] (Goat) (Cat#R1364HRP). No reaction was observed by immunoblotagainst the 25 kDa B subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive againsthuman and mouse NF-Y using 2.0 μL per assay. This product was assayed against NF-Y A subunit peptide in an antibody capture ELISAusing Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) (Cat#R1364HRP)and ABTS substrate. A dilution of 1: 10,000 is suggested from this experiment.
- 限制
- 仅限研究用
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- 浓度
- 1.1 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store lyophilized (preferably in a desiccator) at 2-8 °C and reconstituted (in aliquots) at -20 °C.
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- 抗原
- NFYA (Nuclear Transcription Factor Y, alpha (NFYA))
- 别名
- NFYA (NFYA 产品)
- 别名
- wu:fb16e03 antibody, ik:tdsubc_2b6 antibody, xx:tdsubc_2b6 antibody, NFYA antibody, CBF-A antibody, CBF-B antibody, HAP2 antibody, NF-YA antibody, AA407810 antibody, Cbf-b antibody, SEZ-10 antibody, SEZ10 antibody, nuclear transcription factor Y, alpha antibody, nuclear transcription factor Y subunit alpha antibody, nuclear transcription factor-Y alpha antibody, nfya antibody, NFYA antibody, Nfya antibody
- 背景
- NFY is a transcription factor composed of 3 separate subunits (A,B and C) each of which is required for DNA binding. NFY binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. NFY recognizes a CCAAT motif upstream of gene promoters and is probably involved in the regulation of a variety of genes. Each subunit has remained highly conserved throughout evolution. In fact, homologous yeast subunits can substitute for mammalian NFY in DNA binding assays. The conserved core sequences of NFYB and NFYC contain a 70 aa region similar to the histone fold motif of nucleosomes H2A and H2B. The unique structure and evolutionary conservation of this transcription factor suggests that it plays a fundamental role in the readout of eukaryotic genetic information.Synonyms: Nuclear transcription factor Y subunit alpha
- 基因ID
- 4800
- NCBI登录号
- NP_002496
- UniProt
- P23511
- 途径
- Regulation of Lipid Metabolism by PPARalpha
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