RFX5 抗体 (AA 320-494)
Quick Overview for RFX5 抗体 (AA 320-494) (ABIN116532)
抗原
See all RFX5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 320-494
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特异性
- Anti-RFX5 (aa 320 to 494) may react with unknown minor bands at 65 kDa and 80 kDa. Human and mouse RFX5 from a variety of fibroblast and B-cell lysates. Recognition is of the region between amino acids 320 to 494. Western blot of RFX5 shows a 75 kDa band. A minor band at 68 kDa may be detected which may represent an RFX5 breakdown product or unmodified RFX5.
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纯化方法
- Prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography.
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免疫原
- RFX5 peptide corresponding to a region at amino acids 320 to 494 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
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anti-Regulatory Factor X 5 (RFX5) (AA 1-616) antibody
RFX5 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) (AA 367-616) antibodyRFX5 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) (Internal Region) antibodyVerified RFX5 适用: 人, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) (AA 1-616) antibodyRFX5 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) (AA 516-616) antibodyRFX5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3B8 unconjugated
anti-Regulatory Factor X 5 (RFX5) antibodyRFX5 适用: 人 WB, ELISA, GS, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) antibodyRFX5 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) (N-Term) antibodyRFX5 适用: 人, 犬, 兔, Pig, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) (C-Term) antibodyRFX5 适用: 人, 小鼠, 大鼠, 豚鼠, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) (N-Term) antibodyRFX5 适用: 人, 犬, 兔 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Suitable for for Immunoprecipitation, Immunoblotting, ELISA and supershift assays. Recommended Dilutions: This product was assayed byimmunoblot and found to be reactive against RFX5 (aa 320 to494) from a variety of fibroblast and B-cell lysates at a dilution of 1: 1,000 followed byreaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat). Anti-RFX5 (aa 320 to 494) detects a 75 kDa band by immunoblot for human RFX5. This product was also tested in a gel supershift assay and found to be reactive againstRFX5 complexes using 0.5 to 1.0 l per assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.68 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody (in aliquots) at -20 °C.
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- RFX5 (Regulatory Factor X 5 (RFX5))
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别名
- RFX5
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背景
- RFX5 is the regulatory gene responsible for human MHC class II deficiency. Patients with this disease exhibit a characteristic defect in the binding of a nuclear factor, RFX, to the X box motif of MHC class II promoters. RFX5 is the 75 kDa subunit of the RFX complex which is absolutely essential and highly specific for regulating MHC class II gene expression and control of the immune response. RFX5 is a protein consisting of 616 amino acids.Synonyms: DNA-binding protein RFX5, Regulatory factor X 5
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基因ID
- 5993
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NCBI登录号
- NP_000440
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UniProt
- P48382
抗原
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