Cyclin T1 抗体 (Internal Region)
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北京 101111
Quick Overview for Cyclin T1 抗体 (Internal Region) (ABIN116513)
抗原
See all Cyclin T1 (CCNT1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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特异性
- Antiserum will specifically react with a 85 kDa cyclin T1 protein. No reaction was observed against other related cyclins.
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纯化方法
- Delipidation and defibrination
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免疫原
- Cyclin T1 peptide corresponding to an internal region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
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anti-Cyclin T1 (CCNT1) (AA 375-410), (Middle Region) antibody
CCNT1 适用: 人, 小鼠, 大鼠 WB, IHC, FACS, IF, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin T1 (CCNT1) (AA 253-281) antibodyCCNT1 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB19562 unconjugated
anti-Cyclin T1 (CCNT1) (N-Term) antibodyCCNT1 适用: 人, 小鼠, 大鼠, Cow, 犬, 山羊, 马, 豚鼠, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-Cyclin T1 (CCNT1) (AA 256-285), (Middle Region) antibodyCCNT1 适用: 人, 小鼠 WB, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Cyclin T1 (CCNT1) antibodyKD Validated CCNT1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB150 unconjugated Recombinant Antibody
anti-Cyclin T1 (CCNT1) (AA 1-726) antibodyCCNT1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Cyclin T1 (CCNT1) (AA 220-320) antibodyCCNT1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cyclin T1 (CCNT1) (AA 466-680) antibodyCCNT1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin T1 (CCNT1) (Internal Region) antibodyVerified CCNT1 适用: 人 ELISA, FACS, IF 宿主: 山羊 Polyclonal unconjugated
anti-Cyclin T1 (CCNT1) (N-Term) antibodyCCNT1 适用: 人, 小鼠, 大鼠, Cow, 山羊, 马, Pig, 豚鼠, 兔, 猴, 非洲爪蟾, Bat, Hamster WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Suitable for ELISA, immunoprecipitation, immunohistochemistry, immunoblotting andother immunological methods requiring high titer and specificity. Recommended dilutions: ELISA 1: 1,000 - 1: 5,000Western blot 1: 500 - 1: 2,000Immunohistochemistry 1: 200 - 1: 1,000 (use paraffin embedded tissue).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 75 mg/mL (by refractometry)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M NaCl, pH 7.2, containing 0.01 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C to -80 °C. Avoid multiple freeze/thaw cycles.
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- Cyclin T1 (CCNT1)
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别名
- Cyclin T1
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背景
- Cyclin T1, together with the kinase CDK9, is a component of the transcription elongation factor P-TEFb which binds the human immunodeficiency virus type 1 (HIV-1) transactivator Tat. Tat stimulates human HIV-1 viral transcription elongation. This suggests that cyclin T1/cdk9(PITALRE) is one of the HIV-1 required host cellular cofactors generated during T cell activation. Cyclin T1/cdk9(PITALRE) is shown to interact with Tat to restore Tat activation in HeLa nuclear extracts depleted of P-TEFb. P-TEFb facilitates transcription by phosphorylating the carboxy-terminal domain (CTD) of RNA polymerase II. The cdk9(PITALRE) activity and cyclin T1 are essential for activation of transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion Rev. Cyclin T1 is an exceptionally large cyclin and is therefore a candidate for interactions with regulatory proteins.Synonyms: CCNT1, CycT1, Cyclin-T, Cyclin-T1
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基因ID
- 904
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NCBI登录号
- NP_001231
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UniProt
- O60563
抗原
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