DEFB103A 抗体 (Biotin)
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北京 101111
Quick Overview for DEFB103A 抗体 (Biotin) (ABIN116503)
抗原
See all DEFB103A 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody reacts with Human BD3 (Defensin beta 3). Other species not tested.
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纯化方法
- Affinity Chromatography
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免疫原
- E.coli derived recombinant Human Beta Defensin-3.
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anti-Defensin, beta 103A (DEFB103A) (AA 23-67) antibody
DEFB103A 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Defensin, beta 103A (DEFB103A) (AA 1-67) antibodyDEFB103A 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Defensin, beta 103A (DEFB103A) (AA 6-22) antibodyDEFB103A 适用: 人, 大鼠 WB, EIA, IHC (p), RIA 宿主: 小鼠 Monoclonal L3-18b-E1 unconjugated
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应用备注
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ELISA: Direct: To detect Human BD-3 by direct ELISA (using 100 μL/well antibody solution) thisantibody can be used at a concentration of 1.0 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant HumanBD-3. Sandwich: To detect Human BD-3 by Sandwich ELISA (using 100 μL/well antibody solution)this antibody can be used at a concentration of 0.25-1.0 μg/mL. This biotinylated antibody,used in conjunction with polyclonal anti-human BD-3, allows the detection of at least0.2-0.4 ng/well of recombinant Human BD-3. Western blot: To detect Human BD-3 by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hBD-3 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL
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缓冲液
- BPS, pH 7.2 without preservatives
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
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- DEFB103A (Defensin, beta 103A (DEFB103A))
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别名
- Defensin beta 3
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背景
- Beta Defensin 3 exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. It kills multiresistant S.aureus and vancomycin-resistant E.faecium, but it appears to have no significant hemolytic activity. Beta Defensin 3 is highly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. Low expression is observed in salivary gland, bone marrow, colon, stomach, polyp and larynx, with no expression in small intestine. Expression is induced by infection of bacteria and by interferon gamma. Beta Defensin 3 belongs to the beta-defensin family.Synonyms: BD3, Beta-defensin 103, DEFB103, DEFB103A, DEFB103B, DEFB3, Defensin beta 103, Defensin-like protein, HBD3, HBP3
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基因ID
- 414325
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NCBI登录号
- NP_001075020
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UniProt
- P81534
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途径
- Production of Molecular Mediator of Immune Response
抗原
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