IL-22 抗体 (Biotin)
Quick Overview for IL-22 抗体 (Biotin) (ABIN116491)
抗原
See all IL-22 (IL22) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody recognizes murine IL-22. Not tested in other species.
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纯化方法
- Affinity chromatography
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免疫原
- Highly pure (>98%) recombinant mIL-22 (murine Interleukin-22)
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应用备注
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ELISA: Direct - To detect mIL-22 by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.25 - 1.0 μg/mL, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-22. Sandwich - To detect mIL-22 by sandwich ELISA (using 100 l/well antibody solution),a concentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction with apolyclonal anti-murine IL-22 (PP1226P) as the capture antibody at least 0.2 - 0.4 ng/well ofrecombinant mIL-22 can be detected. Western blot: To detect mIL-22 by Western Blot analysis this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant mIL-22 is 1.5 -3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore with sterile PBS solution containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.
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缓冲液
- BPS, pH 7.2, without preservatives
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized antibody is stable at -20 °C for one year from despatch. The reconstituted antibody can be stored for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.
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有效期
- 12 months
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- IL-22 (IL22) (Interleukin 22 (IL22))
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别名
- Interleukin-22 / IL22
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背景
- IL22 is a cytokine structurally related to IL10 and is upregulated in activated T cells. IL22 signals through a receptor complex that includes CRF2-4 and a new member of the class II cytokine receptor family (also known as the interferon receptor family), IL22R. CRF2-4 has previously been demonstrated to be a functional component of the IL-10 signaling complex. IL22 is able to suppress IL4 production, this may have therapeutic potential, particularly in the treatment of asthma.Synonyms: Cytokine Zcyto18, IL-10-related T-cell-derived-inducible factor, IL-22, ILTIF
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基因ID
- 50929
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NCBI登录号
- NP_058667
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UniProt
- Q9JJY9
抗原
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