IL-22 抗体 (Biotin)
Quick Overview for IL-22 抗体 (Biotin) (ABIN116483)
抗原
See all IL-22 (IL22) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes Human Interleukin-22 (IL-22).
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纯化方法
- Affinity Chromatography.
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免疫原
- Highly pure (>98%) E.coli derived recombinant Human IL-22 (hIL-22).
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应用备注
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ELISADirect: To detect Human IL-22 by direct ELISA (using 100 μL/well) a concentration of approx. 1.0 μg/mL of this antibody is required. The Biotinylated antibody allows the detection of atleast 0.2-0.4 ng/well of recombinant Human IL-22. Sandwich: To detect Human IL-22 by Sandwich ELISA (using 100 μL/well) a concentration of0.25-1.0 μg/mL of this antibody is required. The Biotinylated antibody, in conjuction withanti-Human IL22 (PP1224P1 or PP1224P2) as a capture antibody, allows the detection of atleast 0.2-0.4 ng/well of recombinant Human IL-22. Western blot: To detect Human IL-22 by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Human IL-22 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with sterile PBS solution containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- IL-22 (IL22) (Interleukin 22 (IL22))
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别名
- Interleukin-22 / IL22
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背景
- IL22 is a cytokine structurally related to IL10 and is upregulated in activated T cells. IL22 signals through a receptor complex that includes CRF2-4 and a new member of the class II cytokine receptor family (also known as the interferon receptor family), IL22R. CRF2-4 has previously been demonstrated to be a functional component of the IL-10 signaling complex. IL22 is able to suppress IL4 production, this may have therapeutic potential, particularly in the treatment of asthma.Synonyms: Cytokine Zcyto18, IL-10-related T-cell-derived-inducible factor, IL-22, ILTIF
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基因ID
- 50616
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NCBI登录号
- NP_065386
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UniProt
- Q9GZX6
抗原
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