BMP2 抗体 (Biotin)
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北京 101111
Quick Overview for BMP2 抗体 (Biotin) (ABIN116463)
抗原
See all BMP2 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- The antibody recognizes Human BMP2.Other species not tested.
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纯化方法
- Affinity Chromatography
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免疫原
- Highly pure (>98%) E.coli derived recombinant Human BMP-2.
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应用备注
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ELISA: To detect hBMP-2 by direct ELISA (using 100 l/well antibody solution) aconcentration of 0.15 - 0.30 μg/mL of this antibody is required. This antibody inconjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hBMP-2. Western Blot: To detect hBMP-2 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hBMP-2 is 1.5 -3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C. For prolonged storage of reconstituted antibody solution we strongly recommend addition of a stabilizing protein (e.g. 0.5% BSA) and a preservative (e.g. Thimerosal or Sodium Azide).
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- BMP2 (Bone Morphogenetic Protein 2 (BMP2))
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别名
- BMP2 / BMP2A
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背景
- BMP2 belongs to the transforming growth factor beta (TGFB) superfamily of secreted growth factors. It is a disulfide linked homodimer and induces bone and cartilage formation. In addition to its osteogenic activity, BMP2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. BMPs control fundamental events in early embryonic development, organogenesis and adult tissue homeostasis.Synonyms: BMP-2, BMP-2A, Bone morphogenetic protein 2
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基因ID
- 650
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NCBI登录号
- NP_001191
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UniProt
- P12643
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途径
- Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation
抗原
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