Prokineticin 1 抗体
Quick Overview for Prokineticin 1 抗体 (ABIN116413)
抗原
See all Prokineticin 1 (Prok1) 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Affinity chromatography.
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免疫原
- Highly pure (>98%) recombinant hEG-VEGF.
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应用备注
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Neutralization. ELISA: To detect hEG-VEGF by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hEG-VEGF. Western Blot: To detect hEG-VEGF by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hEG-VEGF is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of > 0.2 mg/mL.
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缓冲液
- 0.5 X PBS pH 7.4 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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