CXCL16 抗体
Quick Overview for CXCL16 抗体 (ABIN116405)
抗原
See all CXCL16 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody recognizes Human CXCL16. Other species not tested.
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纯化方法
- Affinity Chromatography employing immobilized hCXCL16 matrix
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免疫原
- Highly pure (> 98%) E.coli derived recombinant Human CXCL16
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应用备注
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Indirect ELISA: To detect hCXCL16 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith compatible secondary reagents, allows the detection of 0.2-0.4 ng/well ofrecombinant hCXCL16. Sandwich ELISA: To detect hCXCL16 by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antibody, inconjunction with a biotinylated anti-Human CXCL16 (PP1204B1 or PP1204B2) as a detectionantibody, allows the detection of 0.2-0.4 ng/well of recombinant hCXCL16. Western Blot: To detect hCXCL16 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. The detection limit for recombinant hCXCL16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry: The antibody stained FFPE Sections of human colorectaladenocarcinoma tissue. An HRP-labelled polymer detection system was used with a DAB chromogen). Antigenretrieval is not required for these conditions. Working Dilution: 0.5 μg/mL, overnight incubation at 4 °C.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL
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缓冲液
- PBS, pH 7.2 without preservatives
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储存液
- Without preservative
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注意事项
- Centrifuge vial prior to opening! This product is photosensitive and should be protected from light
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储存条件
- 4 °C
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储存方法
- Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
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: "CXCL16 is expressed in podocytes and acts as a scavenger receptor for oxidized low-density lipoprotein." in: The American journal of pathology, Vol. 174, Issue 6, pp. 2061-72, (2009) (PubMed).
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: "CXCL16 is expressed in podocytes and acts as a scavenger receptor for oxidized low-density lipoprotein." in: The American journal of pathology, Vol. 174, Issue 6, pp. 2061-72, (2009) (PubMed).
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- CXCL16 (Chemokine (C-X-C Motif) Ligand 16 (CXCL16))
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别名
- CXCL16
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背景
- CXCL16 acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo. It is a type I membrane protein (Potential). Also exists as a soluble form. Expressed in spleen, lymph nodes, lung, kidney, small intestine and thymus. Weak expression in heart and liver and no expression in brain and bone marrow.Synonyms: C-X-C motif chemokine 16, SCYB16, SR-PSOX, SRPSOX, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, Small-inducible cytokine B16, Transmembrane chemokine CXCL16
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基因ID
- 58191
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NCBI登录号
- NP_001094282
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UniProt
- Q9H2A7
抗原
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