beta 2 Defensin 抗体 (Biotin)
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- 抗原 See all beta 2 Defensin (BD-2) 抗体
- beta 2 Defensin (BD-2) (Defensin beta 2 (BD-2))
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This beta 2 Defensin antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- This antibody recognizes Human BD2 (Defensin beta 2). Other species not tested.
- 纯化方法
- Immunoaffinity Chromatography.
- 免疫原
- Highly pure (>98%) recombinant Human BD-2 (hBD-2)
- Top Product
- Discover our top product BD-2 Primary Antibody
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- 应用备注
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Direct ELISA: To detect hBD-2 by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hBD-2. Sandwich ELISA: To detect hBD-2 by Sandwich ELISA (using 100 μL/well antibody solution)a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with a compatible polyclonal Anti-Human BD-2 (PP1125P1 orPP1125P2) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant hBD-2. Western Blot: To detect hBD-2 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hBD-2 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
- 缓冲液
- PBS, pH 7.2 without preservatives
- 储存液
- Without preservative
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized antibody is stable at -20 °C for one year from date of despatch. The reconstituted solution can be stored at 2-8 °C for two weeks. Frozen aliquots of this solution are stable for six months when kept at -20 °C.
- 有效期
- 12 months
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- 抗原
- beta 2 Defensin (BD-2) (Defensin beta 2 (BD-2))
- 别名
- Defensin beta 2 (BD-2 产品)
- 别名
- BD-2 antibody, BD-4 antibody, DEFB-4 antibody, hBD-4 antibody, BD2 antibody, DEFB2 antibody, defensin beta 2 antibody, defensin beta 104B antibody, beta defensin 2 antibody, Defb2 antibody, DEFB104B antibody, SBD2 antibody
- 背景
- This antibiotic peptide is locally regulated by inflammation. Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. hBD-2 is a antimicrobial peptide, which belongs to the distinct family of beta-defensins.Synonyms: BD-2, Beta-defensin 2, Beta-defensin 4A, DEFB102, DEFB2, DEFB4A, DEFB4B, Skin-antimicrobial peptide 1, hBD-2
- 基因ID
- 100289462
- NCBI登录号
- NP_001192195
- UniProt
- O15263
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