beta 2 Defensin 抗体 (Biotin)
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Quick Overview for beta 2 Defensin 抗体 (Biotin) (ABIN116359)
抗原
See all beta 2 Defensin (BD-2) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody recognizes Human BD2 (Defensin beta 2). Other species not tested.
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纯化方法
- Immunoaffinity Chromatography.
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免疫原
- Highly pure (>98%) recombinant Human BD-2 (hBD-2)
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anti-Defensin beta 2 (BD-2) (AA 24-64) antibody
BD-2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Defensin beta 2 (BD-2) (AA 21-71) antibodyBD-2 适用: 小鼠 IF (cc), IF (p), ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Defensin beta 2 (BD-2) (AA 21-71) antibodyBD-2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Defensin beta 2 (BD-2) (AA 21-71) antibodyBD-2 适用: 人, 大鼠 WB, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Defensin beta 2 (BD-2) (AA 23-63) antibodyBD-2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Defensin beta 2 (BD-2) (AA 4-41) antibodyBD-2 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal L12-4C-C2 unconjugated
anti-Defensin beta 2 (BD-2) (AA 4-41) antibodyBD-2 适用: 人 WB, IHC (p), EIA 宿主: 小鼠 Monoclonal L12-4C-C2 unconjugated
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应用备注
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Direct ELISA: To detect hBD-2 by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hBD-2. Sandwich ELISA: To detect hBD-2 by Sandwich ELISA (using 100 μL/well antibody solution)a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with a compatible polyclonal Anti-Human BD-2 (PP1125P1 orPP1125P2) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant hBD-2. Western Blot: To detect hBD-2 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hBD-2 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized antibody is stable at -20 °C for one year from date of despatch. The reconstituted solution can be stored at 2-8 °C for two weeks. Frozen aliquots of this solution are stable for six months when kept at -20 °C.
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有效期
- 12 months
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- beta 2 Defensin (BD-2) (Defensin beta 2 (BD-2))
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别名
- Defensin beta 2
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背景
- This antibiotic peptide is locally regulated by inflammation. Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. hBD-2 is a antimicrobial peptide, which belongs to the distinct family of beta-defensins.Synonyms: BD-2, Beta-defensin 2, Beta-defensin 4A, DEFB102, DEFB2, DEFB4A, DEFB4B, Skin-antimicrobial peptide 1, hBD-2
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基因ID
- 100289462
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NCBI登录号
- NP_001192195
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UniProt
- O15263
抗原
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