alpha Defensin 1 抗体
Quick Overview for alpha Defensin 1 抗体 (ABIN116329)
抗原
See all alpha Defensin 1 (DEFA1) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- The antibody recognizes Human NP-1.
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Affinity Chromatography employing immobilized hNP-1 matrix
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免疫原
- Highly pure (>98%) recombinant Human NP-1
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anti-Defensin, alpha 1 (DEFA1) (AA 1-94) antibody
DEFA1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Defensin, alpha 1 (DEFA1) (AA 20-94) antibodyDEFA1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Defensin, alpha 1 (DEFA1) (AA 20-93) antibodyDEFA1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Defensin, alpha 1 (DEFA1) (AA 1-94) antibodyDEFA1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Defensin, alpha 1 (DEFA1) antibodyDEFA1 适用: 人 WB, IHC, ELISA, Neut, IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Defensin, alpha 1 (DEFA1) antibodyDEFA1 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Defensin, alpha 1 (DEFA1) (AA 28-62) antibodyDEFA1 适用: 人 WB 宿主: 兔 Polyclonal RB57879 unconjugated
anti-Defensin, alpha 1 (DEFA1) (Internal Region) antibodyVerified DEFA1 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
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应用备注
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ELISA. Indirect: To detect hNP-1 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well ofrecombinant hNP-1. Sandwich: To detect hNP-1 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith a biotinylated Anti-Human NP-1 (PP1112B) as a detection antibody, allows thedetection of at least 0.2-0.4 ng/well of recombinant hNP-1. Western Blot: To detect hNP-1 by Western Blot analysis this antibody can be used at a concentration of0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant hNP-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Neutralization: To yield one-half maximal inhibition (ND50) of the biological activity of hNP-1 (100 ng/mL),a concentration of 0.40-0.60 μg/mL of this antibody has been used with previous lots.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening.
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized antibody is stable for one year from despatch at -20 °C. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
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有效期
- 12 months
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- alpha Defensin 1 (DEFA1) (Defensin, alpha 1 (DEFA1))
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别名
- Defensin alpha 1
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背景
- Defensins are a group of cyclic peptides containing 29-35 amino acids, which comprise approximately 30 % of the total protein content of neutrophil azurophilic granules. Various functions have been reported for the defensins, including antibacterial and antifungal activity and chemotactic activity for monocytes.Synonyms: DEF-1, DEF-3, DEF-A1, DEF-A2, DEF-A3, DEF1, DEF3, DEFA-1, DEFA-2, DEFA-3, DEFA1, DEFA2, DEFA3, Defensin alpha 1, Defensin alpha 3, HNP-1, HNP-3, HNP1, HNP3, HP-1, HP-3, HP1, HP3, MRS, Neutrophil defensin 1, Neutrophil defensin 3
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基因ID
- 1667
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NCBI登录号
- NP_001035965
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UniProt
- P59665
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途径
- Cellular Response to Molecule of Bacterial Origin
抗原
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