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alpha Defensin 1 抗体

DEFA1 适用: 人 WB, EIA, Func 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN116329
发货至: 中国
  • 抗原 See all alpha Defensin 1 (DEFA1) 抗体
    alpha Defensin 1 (DEFA1) (Defensin, alpha 1 (DEFA1))
    适用
    • 26
    • 2
    • 2
    宿主
    • 16
    • 9
    • 2
    山羊
    克隆类型
    • 25
    • 2
    多克隆
    标记
    • 13
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This alpha Defensin 1 antibody is un-conjugated
    应用范围
    • 15
    • 12
    • 6
    • 6
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)
    特异性
    The antibody recognizes Human NP-1.
    交叉反应 (详细)
    Species reactivity (tested):Human.
    纯化方法
    Affinity Chromatography employing immobilized hNP-1 matrix
    免疫原
    Highly pure (>98%) recombinant Human NP-1
    Top Product
    Discover our top product DEFA1 Primary Antibody
  • 应用备注
    ELISA. Indirect: To detect hNP-1 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well ofrecombinant hNP-1. Sandwich: To detect hNP-1 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith a biotinylated Anti-Human NP-1 (PP1112B) as a detection antibody, allows thedetection of at least 0.2-0.4 ng/well of recombinant hNP-1. Western Blot: To detect hNP-1 by Western Blot analysis this antibody can be used at a concentration of0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant hNP-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Neutralization: To yield one-half maximal inhibition (ND50) of the biological activity of hNP-1 (100 ng/mL),a concentration of 0.40-0.60 μg/mL of this antibody has been used with previous lots.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
    缓冲液
    PBS, pH 7.2 without preservatives.
    储存液
    Without preservative
    注意事项
    Avoid repeated freezing and thawing. Centrifuge vial prior to opening.
    储存条件
    4 °C/-20 °C
    储存方法
    The lyophilized antibody is stable for one year from despatch at -20 °C. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
    有效期
    12 months
  • 抗原
    alpha Defensin 1 (DEFA1) (Defensin, alpha 1 (DEFA1))
    别名
    Defensin alpha 1 (DEFA1 产品)
    背景
    Defensins are a group of cyclic peptides containing 29-35 amino acids, which comprise approximately 30 % of the total protein content of neutrophil azurophilic granules. Various functions have been reported for the defensins, including antibacterial and antifungal activity and chemotactic activity for monocytes.Synonyms: DEF-1, DEF-3, DEF-A1, DEF-A2, DEF-A3, DEF1, DEF3, DEFA-1, DEFA-2, DEFA-3, DEFA1, DEFA2, DEFA3, Defensin alpha 1, Defensin alpha 3, HNP-1, HNP-3, HNP1, HNP3, HP-1, HP-3, HP1, HP3, MRS, Neutrophil defensin 1, Neutrophil defensin 3
    基因ID
    1667
    NCBI登录号
    NP_001035965
    UniProt
    P59665
    途径
    Cellular Response to Molecule of Bacterial Origin
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