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GM-CSF 抗体

This 兔 多克隆 antibody specifically detects GM-CSF in WB, EIA, IHC (p) 和 Func. It exhibits reactivity toward 人.
产品编号 ABIN116305
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for GM-CSF 抗体 (ABIN116305)

抗原

See all GM-CSF (CSF2) 抗体
GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

适用

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克隆类型

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多克隆

标记

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This GM-CSF antibody is un-conjugated

应用范围

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Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)
  • 特异性

    This antibody recognizes Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF). Other species not tested.

    纯化方法

    Affinity Chromatography employing immobilized hGM-CSF matrix

    免疫原

    Highly pure (>98%) E.coli derived recombinant Human GM-CSF (Human Granulocyte Macrophage Colony Stimulating Factor)
  • 应用备注

    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hGMCSF (1.0 ng/mL), a concentration of 0.30-0.50 μg/mL of this antibody is required. ELISA: To detect hGM CSF by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant hGM CSF. Western Blot: To detect hGM CSF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hGM CSF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

    缓冲液

    PBS, pH 7.2 without preservatives

    储存液

    Without preservative

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
  • 抗原

    GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

    别名

    GM-CSF

    背景

    The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.Synonyms: CSF2, Colony stimulating factor 2, GMCSF, Granulocyte-macrophage colony-stimulating factor, Molgramostin, Sargramostim

    基因ID

    1437

    NCBI登录号

    NP_000749

    UniProt

    P04141

    途径

    JAK/STAT Signaling, Cellular Response to Molecule of Bacterial Origin
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