CNTF 抗体 (Biotin)
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Quick Overview for CNTF 抗体 (Biotin) (ABIN116299)
抗原
See all CNTF 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Ciliary Neurotrophic Factor (CNTF)
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纯化方法
- Affinity chromatography.
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免疫原
- Highly pure (>98%) recombinant hCNTF (human CTNF).
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应用备注
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ELISA: To detect hCNTF by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hCNTF. Western Blot: To detect hCNTF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hCNTF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody at 2-8 °C or at -20 °C for longer.
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- CNTF (Ciliary Neurotrophic Factor (CNTF))
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别名
- CNTF
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背景
- Ciliary neurotrophic factor (CNTF) is expressed in glial cells within the central and peripheral nervous systems. CNTF stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types such as sensory, sympathetic, ciliary and motor neurons. CNTF itself lacks a classical signal peptide sequence of a secreted protein, but is thought to convey its cytoprotective effects after release from adult glial cells by some mechanism induced by injury. In addition to its neuronal actions, CNTF also acts on non neuronal cells such as glia, hepatocytes, skeletal muscle, embryonic stem cells and bone marrow stromal cells.Synonyms: Ciliary Neurotrophic Factor
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基因ID
- 1270
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NCBI登录号
- NP_000605
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UniProt
- P26441
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途径
- JAK/STAT Signaling
抗原
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