CXCL10 抗体 (Biotin)
Quick Overview for CXCL10 抗体 (Biotin) (ABIN116269)
抗原
See all CXCL10 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- Reacts with Interferon-gamma Inducible Protein-10.
-
纯化方法
- Affinity Chromatography
-
免疫原
- Highly pure (>98%) E.coli derived recombinant Mouse IP-10.
-
-
-
-
应用备注
-
Direct ELISA: To detect Mouse IP-10 by Direct ELISA (using 100 ml/well antibody solution)this antibody can be used at a concentration of approx. 1.0 μg/mL. This Biotinylated antibodyallows the detection of at least 0.2-0.4 ng/well of recombinant Mouse IP-10. Sandwich ELISA: To detect Mouse IP-10 by Sandwich ELISA (using 100 ml/well antibodysolution), a concentration of 0.25-1.0 μg/mL of this antibody is required. This Biotinylatedantibody, in conjuction with anti Mouse IP10 antibody (cat. PP1083P) as a Captureantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Mouse IP-10. Western Blot: To detect Mouse IP-10 by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Mouse IP-10 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
溶解方式
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
-
缓冲液
- PBS, pH 7.2 without preservatives
-
储存液
- Without preservative
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C/-20 °C
-
储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
-
-
- CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))
-
别名
- CXCL10 / IP-10
-
背景
- Interferon-gamma-inducible 10 kD protein (IP-10), is a CXC chemokine with chemoattractant properties for CD4-positive T cells and inhibits early normal and leukemic hemopoietic progenitor proliferation. IP-10 is produced by a wide variety of cell types ranging from neutrophils and monocytes to hepatocytes, endothelial cells and keratinocytes. The cytokine is reported to be involved in a scala of inflammatory pathologies such as HIV encephalitis, cutaneous T cell lymphoma, chronic hepatitis and acute anterior uveitis. Various observations strongly suggest a role for the CXC chemokines IL-8 and IP-10 in the regulation of angiogenic activity in cancer and in idiopathic pulmonary fibrosis.Synonyms: 10 kDa interferon-gamma-induced protein, C-X-C motif chemokine 10, Gamma-IP10, INP10, SCYB10, Small-inducible cytokine B10
-
基因ID
- 15945
-
NCBI登录号
- NP_067249
-
UniProt
- P17515
抗原
-