CXCL2 抗体
Quick Overview for CXCL2 抗体 (ABIN116267)
抗原
See all CXCL2 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects Mouse MIP-2. Other species not tested.
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纯化方法
- Affinity Chromatography employing immobilized murine mMIP-2 matrix
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免疫原
- Highly pure (> 98%) E.coli derived recombinant Murine MIP-2
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anti-Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (AA 34-107) antibody
CXCL2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (AA 28-100) antibodyCXCL2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (AA 1-100) antibodyCXCL2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2D10 unconjugated
anti-Chemokine (C-X-C Motif) Ligand 2 (CXCL2) antibodyCXCL2 适用: 人 WB, ELISA, Func, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Ligand 2 (CXCL2) antibody (Biotin)CXCL2 适用: 大鼠 WB, ELISA, Neut 宿主: 兔 Polyclonal Biotin
anti-Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (AA 35-107) antibodyCXCL2 适用: 马 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (AA 32-100) antibodyCXCL2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (AA 1-107) antibodyCXCL2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity of mMIP-2 (15.00 ng/mL), a concentration of 0.52-0.90 μg/mL of this antibody is required. ELISA: Indirect: Using 100 μL/well antibody solution, a concentration of 0.5 - 2.0 μg/mL isrequired. In conjunction with compatible secondary reagents, it allows the detection of atleast 0.2 - 0.4 ng/well of recombinant mMIP-2. Sandwich: Using 100 μL/well antibody solution, a concentration of 0.5 - 2.0 μg/mL isrequired. In conjunction with Biotinylated Anti-Murine MIP-2 (PP1082B) as a detectionantibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMIP-2. Western Blot: 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents thedetection limit for recombinant mMIP-2 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store (in aliquots) at -20 °C.
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- CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))
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别名
- CXCL2 / MIP2A
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背景
- GRO beta is a member of the CXC, or chemokine class. It contains the ELR domain immediately preceding the first cysteine residue near the amino terminus. Other chemokines in this group include IL8, GRO alpha/beta/gamma, mouse KC, ENA78, GCP2, PBP/CTAPIII/beta TG/NAP2. These chemokines act primarily on neutrophils as chemoattractants and activators, including neutrophil degradation with release of myloperoxidase and other enzymes. GRO beta was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. GRO beta is an approximately 8 kDa polypeptide of 73 amino acids. The precursor form of GRO beta consists of 100 amino acids. To generate the mature GRO beta, the precursor cleaves its amino terminal 27 amino acids. GRO beta shows 60 % amino acid homology to human GRO alpha and GRO gamma.Synonyms: C-X-C motif chemokine 2, GRO2, GROB, Gro-beta, Growth-regulated protein beta, MIP-2A, MIP2-alpha, Macrophage inflammatory protein 2-alpha, SCYB2
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基因ID
- 20310
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NCBI登录号
- NP_033166
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UniProt
- P10889
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途径
- Cellular Response to Molecule of Bacterial Origin
抗原
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