CCL5 抗体 (Biotin)
Quick Overview for CCL5 抗体 (Biotin) (ABIN116261)
抗原
See all CCL5 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- The antibody reacts with Murine RANTES.
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交叉反应 （详细）
- Species reactivity (tested):Mouse.
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纯化方法
- Affinity Chromatography.
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免疫原
- Highly pure (>98%) recombinant Murine RANTES. Remarks: Molecular Weight: ~150 kDa
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anti-Chemokine (C-C Motif) Ligand 5 (CCL5) (AA 24-91) antibody
CCL5 适用: 犬 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 5 (CCL5) (AA 24-91) antibodyCCL5 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 5 (CCL5) (AA 62-91) antibodyCCL5 适用: 人, 小鼠, 豚鼠, Pig ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 5 (CCL5) (AA 24-91) antibodyCCL5 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 5 (CCL5) antibodyCCL5 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 5 (CCL5) antibodyCCL5 适用: 人 WB, ELISA, Func, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 5 (CCL5) antibody (Biotin)CCL5 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal Biotin
anti-Chemokine (C-C Motif) Ligand 5 (CCL5) (AA 24-91) antibodyCCL5 适用: 猕猴 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 5 (CCL5) (AA 24-91) antibodyCCL5 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- ELISADirect: To detect Murine RANTES by Direct ELISA (using 100 μL/well antibody solution) aconcentration of approx. 1.0 μg/mL of this antibody is required. This Biotinylated polyclonalpurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant Murine RANTES. Sandwich: To detect Murine RANTES by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This Biotinylatedpolyclonal antibody, in conjunction with Anti Murine RANTES (PP1081P1 or PP1081P2) as acapture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinantmRANTES. Western BlotTo detect Murine RANTES by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mRANTES is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
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限制
- 仅限研究用
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溶解方式
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
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别名
- RANTES / CCL5
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背景
- RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.Synonyms: C-C motif chemokine 5, D17S136E, E °CP, Eosinophil-chemotactic cytokine, SCYA5, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, cell-specific protein P228
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基因ID
- 20304
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NCBI登录号
- NP_038681
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UniProt
- P30882
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration
抗原
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