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Thrombopoietin 抗体

The 山羊 多克隆 anti-Thrombopoietin antibody is suitable to detect Thrombopoietin in samples from 人. It has been validated for WB, EIA 和 Func.
产品编号 ABIN116255
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Thrombopoietin 抗体 (ABIN116255)

抗原

See all Thrombopoietin (THPO) 抗体
Thrombopoietin (THPO)

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This Thrombopoietin antibody is un-conjugated

应用范围

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Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)
  • 特异性

    This antibody reacts with Thrombopoietin.

    纯化方法

    Affinity chromatography.

    免疫原

    Highly purified recombinant human TPO.
  • 应用备注

    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhTPO (5.00 ng/mL), a concentration of 4.2-6.3 μg/mL of this antibody is required. ELISA: (Indirect): To detect hTPO by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hTPO. (Sandwich): To detect hTPO by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human TPO (PP1074B) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTPO. Western Blot: To detect hTPO by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTPO is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

    缓冲液

    PBS, pH 7.2 without preservatives.

    储存液

    Without preservative

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • 抗原

    Thrombopoietin (THPO)

    别名

    Thrombopoietin

    背景

    Synonyms: C-mpl ligand, MGDF, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, THPO

    基因ID

    7066

    NCBI登录号

    NP_000451

    UniProt

    P40225

    途径

    JAK/STAT Signaling, Hormone Activity
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