Thrombopoietin 抗体
Quick Overview for Thrombopoietin 抗体 (ABIN116255)
抗原
See all Thrombopoietin (THPO) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody reacts with Thrombopoietin.
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纯化方法
- Affinity chromatography.
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免疫原
- Highly purified recombinant human TPO.
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anti-Thrombopoietin (THPO) (AA 22-259) antibody
THPO 适用: 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Thrombopoietin (THPO) (AA 22-349) antibodyTHPO 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Thrombopoietin (THPO) antibodyTHPO 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 1B11 unconjugated
anti-Thrombopoietin (THPO) (AA 21-120) antibodyTHPO 适用: 人, 大鼠, 小鼠 WB, ELISA, ICC, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Thrombopoietin (THPO) (AA 25-298) antibodyTHPO 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Thrombopoietin (THPO) (AA 24-266) antibodyTHPO 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Thrombopoietin (THPO) antibodyTHPO 适用: 人 WB, EIA 宿主: 小鼠 Monoclonal 1B11 unconjugated
anti-Thrombopoietin (THPO) (AA 22-194) antibodyTHPO 适用: 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Thrombopoietin (THPO) (AA 251-300) antibodyTHPO 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Thrombopoietin (THPO) (AA 1-349) antibodyTHPO 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhTPO (5.00 ng/mL), a concentration of 4.2-6.3 μg/mL of this antibody is required. ELISA: (Indirect): To detect hTPO by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hTPO. (Sandwich): To detect hTPO by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human TPO (PP1074B) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTPO. Western Blot: To detect hTPO by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTPO is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- Thrombopoietin (THPO)
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别名
- Thrombopoietin
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背景
- Synonyms: C-mpl ligand, MGDF, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, THPO
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基因ID
- 7066
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NCBI登录号
- NP_000451
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UniProt
- P40225
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途径
- JAK/STAT Signaling, Hormone Activity
抗原
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