LTA 抗体
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北京 101111
Quick Overview for LTA 抗体 (ABIN116247)
抗原
See all LTA 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody reacts with Tumor Necrosis Factor beta.
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纯化方法
- Affinity chromatography.
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免疫原
- Highly pure (>98%) recombinant hTNF-beta (human Tumor Necrosis Factor-beta).
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anti-Lymphotoxin-alpha (LTA) (AA 49-202) antibody
LTA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lymphotoxin-alpha (LTA) (AA 36-205) antibodyLTA 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C4 unconjugated
anti-Lymphotoxin-alpha (LTA) (Internal Region) antibodyLTA 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lymphotoxin-alpha (LTA) (AA 1-205) antibodyLTA 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Lymphotoxin-alpha (LTA) (AA 36-205) antibodyLTA 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lymphotoxin-alpha (LTA) (AA 35-191) antibodyLTA 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lymphotoxin-alpha (LTA) (AA 46-72) antibodyLTA 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB21239 unconjugated
anti-Lymphotoxin-alpha (LTA) (Middle Region) antibodyLTA 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Lymphotoxin-alpha (LTA) (AA 116-203) antibodyLTA 适用: 斑马鱼 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Lymphotoxin-alpha (LTA) (Internal Region) antibodyLTA 适用: 人, 大鼠, 小鼠, 猴 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhTNF-beta (0.12 ng/mL), a concentration of 0.002-0.004 μg/mL of this antibody is required. ELISA: (Indirect): To detect hTNF-β by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2 - 0.4 ng/well of recombinant hTNF-β. (Sandwich): To detect hTNF-β by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human TNF-β (PP1072B) as a detectionantibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTNF-β. Western Blot: To detect hTNF-β by Western Blot analysis this antibody can be used at aconcentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTNF-β is 1.5-3.0 ng/lane, under either reducingnon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- LTA (Lymphotoxin-alpha (LTA))
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别名
- TNF-beta / Lymphotoxin-alpha
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物质类
- Chemical
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背景
- Synonyms: LT-alpha, LTA, TNFB, TNFSF1, Tumor necrosis factor ligand superfamily member 1
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基因ID
- 4049
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NCBI登录号
- NP_000586
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UniProt
- P01374
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
抗原
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