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LTA 抗体 (Biotin)

This anti-LTA antibody is a 兔 多克隆 antibody detecting LTA in WB 和 EIA. Suitable for .
产品编号 ABIN116245
发货至: 中国

Quick Overview for LTA 抗体 (Biotin) (ABIN116245)

抗原

See all LTA 抗体
LTA (Lymphotoxin-alpha (LTA))

适用

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宿主

  • 86
  • 33
  • 4
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克隆类型

  • 86
  • 38
  • 1
多克隆

标记

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This LTA antibody is conjugated to Biotin

应用范围

  • 92
  • 55
  • 40
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  • 10
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Western Blotting (WB), Enzyme Immunoassay (EIA)
  • 特异性

    This antibody reacts with Tumor Necrosis Factor beta.

    纯化方法

    Affinity chromatography.

    免疫原

    Highly pure (>98%) recombinant hTNF-beta.
  • 应用备注

    ELISA: (Direct): To detect hTNF-β by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hTNF-β. (Sandwich): To detect hTNF-β by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with Polyclonal Anti-Human TNF-β (PP1072P1/PP1072P2) as acapture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTNF-β. Western Blot: To detect hTNF-β by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTNF-β is 1.5- 3.0 ng/lane, under either reducing ornon-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

    缓冲液

    PBS, pH 7.2 without preservatives.

    储存液

    Without preservative

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • 抗原

    LTA (Lymphotoxin-alpha (LTA))

    别名

    TNF-beta / Lymphotoxin-alpha

    物质类

    Chemical

    背景

    Synonyms: LT-alpha, LTA, TNFB, TNFSF1, Tumor necrosis factor ligand superfamily member 1

    基因ID

    4049

    NCBI登录号

    NP_000586

    UniProt

    P01374

    途径

    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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