CCL5 抗体
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北京 101111
Quick Overview for CCL5 抗体 (ABIN116219)
抗原
See all CCL5 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes Human RANTES.
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纯化方法
- Affinity Chromatography employing immobilized hRANTES matrix.
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免疫原
- Highly pure (>98%) derived recombinant Human RANTES.
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应用备注
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhRANTES (100 ng/mL), a concentration of 5.0 - 7.0 μg/mL of this antibody is required. ELISA: To detect hRANTES by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5g/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 -0.4 ng/well of recombinant hRANTES. Western Blot: To detect hRANTES by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hRANTES is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
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别名
- RANTES / CCL5
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背景
- Rantes, also known as CCL5, is the protein classified as a chemotactic cytokine or chemokine. This protein is a chemotactic for blood monocytes, memory T helper cells, eosinophils and basophils, and plays an active role in recruiting leukocytes into inflammatory sites. Rantes also induces the proliferation and activation of certain natural-killer (NK) cells to form CHAK (CC-Chemokine-activated killer) cells. It is also an HIV-suppressive factor released from CD8+ T cells.Synonyms: C-C motif chemokine 5, D17S136E, E °CP, Eosinophil-chemotactic cytokine, SCYA5, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, cell-specific protein P228
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基因ID
- 6352
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NCBI登录号
- NP_002976
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UniProt
- P13501
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration
抗原
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