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PF4 抗体

This anti-PF4 antibody is a 兔 多克隆 antibody detecting PF4 in WB, IHC (p), EIA 和 Func. Suitable for 人.
产品编号 ABIN116215
发货至: 中国

Quick Overview for PF4 抗体 (ABIN116215)

抗原

See all PF4 抗体
PF4 (Platelet Factor 4 (PF4))

适用

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宿主

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克隆类型

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多克隆

标记

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This PF4 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)
  • 特异性

    This antibody reacts with Human Platelet Factor 4 (PF-4).

    纯化方法

    Affinity Chromatography employing immobilized Human PF-4 matrix

    免疫原

    Highly pure (>98%) E.coli derived recombinant human Platelet Factor-4 (hPF-4)
  • 应用备注

    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhPF?4 (50 ng/mL), a concentration of 1.75-3.0 μg/mL of this antibody is required. ELISA: To detect hPF?4 by direct ELISA (using 100l/well antibody solution) a concentrationof at least 0.5g/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/wellof recombinant hPF?4. Western Blot: To detect hPF?4 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hPF?4 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Centrifuge vial prior to opening.Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

    缓冲液

    PBS, pH 7.2 without preservatives

    储存液

    Without preservative

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
  • 抗原

    PF4 (Platelet Factor 4 (PF4))

    别名

    Platelet Factor 4 / PF4

    背景

    Synonyms: C-X-C motif chemokine 4, CXCL4, Iroplact, Oncostatin-A, PF-4, SCYB4

    基因ID

    5196

    NCBI登录号

    NP_002610

    UniProt

    P02776
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