PF4 抗体 (Biotin)
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北京 101111
Quick Overview for PF4 抗体 (Biotin) (ABIN116213)
抗原
See all PF4 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Human Platelet Factor 4 (hPF-4).
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纯化方法
- Affinity chromatography.
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免疫原
- Highly pure (>98%) recombinant hPF-4 (human PF- 4).
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应用备注
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ELISA: To detect hPF-4 by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hPF-4. Western Blot: To detect hPF-4 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hPF-4 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody at 2-8 °C for one month or in aliquots at -20 °C for longer.
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