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Oncostatin M 抗体

The 兔 多克隆 anti-Oncostatin M antibody is suitable to detect Oncostatin M in samples from 人. It has been validated for WB, IHC (p), EIA 和 Func.
产品编号 ABIN116204
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Oncostatin M 抗体 (ABIN116204)

抗原

See all Oncostatin M (OSM) 抗体
Oncostatin M (OSM)

适用

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宿主

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克隆类型

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多克隆

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This Oncostatin M antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)
  • 特异性

    Human Oncostatin M.

    纯化方法

    Affinity chromatography.

    免疫原

    Highly pure (>98%) recombinant Oncostatin m:
  • 应用备注

    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofOncostatin M (10 ng/mL), a concentration of 0.1 - 0.175 μg/mL of this antibody is required. Immunohistochemistry: We recommend use of 1 μg/mL as a starting dilution. Paraffinsections may require pretreatment like Proteinase K or Trypsin predigestion to improvestaining. ELISA: To detect Oncostatin M by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant Oncostatin M. Western Blot: use a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatiblesecondary reagents the detection limit for recombinant Oncostatin M is 1.5 - 3.0 ng/lane,under either reducing or non-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

    缓冲液

    PBS, pH 7.2 without preservatives.

    储存液

    Without preservative

    注意事项

    Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • 抗原

    Oncostatin M (OSM)

    别名

    Oncostatin-M

    背景

    Synonyms: OSM

    基因ID

    5008

    NCBI登录号

    NP_065391

    UniProt

    P13725

    途径

    JAK/STAT Signaling, Negative Regulation of Hormone Secretion
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