Oncostatin M 抗体
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北京 101111
Quick Overview for Oncostatin M 抗体 (ABIN116204)
抗原
See all Oncostatin M (OSM) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Human Oncostatin M.
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纯化方法
- Affinity chromatography.
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免疫原
- Highly pure (>98%) recombinant Oncostatin m:
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应用备注
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofOncostatin M (10 ng/mL), a concentration of 0.1 - 0.175 μg/mL of this antibody is required. Immunohistochemistry: We recommend use of 1 μg/mL as a starting dilution. Paraffinsections may require pretreatment like Proteinase K or Trypsin predigestion to improvestaining. ELISA: To detect Oncostatin M by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant Oncostatin M. Western Blot: use a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatiblesecondary reagents the detection limit for recombinant Oncostatin M is 1.5 - 3.0 ng/lane,under either reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- Oncostatin M (OSM)
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别名
- Oncostatin-M
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背景
- Synonyms: OSM
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基因ID
- 5008
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NCBI登录号
- NP_065391
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UniProt
- P13725
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途径
- JAK/STAT Signaling, Negative Regulation of Hormone Secretion
抗原
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