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Oncostatin M 抗体

OSM 适用: 人 WB, IHC (p), EIA, Func 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN116203
发货至: 中国
  • 抗原 See all Oncostatin M (OSM) 抗体
    Oncostatin M (OSM)
    适用
    • 56
    • 10
    • 9
    宿主
    • 59
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    • 1
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    克隆类型
    • 62
    • 7
    多克隆
    标记
    • 36
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    • 1
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    This Oncostatin M antibody is un-conjugated
    应用范围
    • 55
    • 31
    • 13
    • 13
    • 9
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    • 6
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    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)
    特异性
    Human Oncostatin M.
    纯化方法
    Affinity Chromatography employing immobilized Human Oncostatin M matrix
    免疫原
    Highly pure (>98%) E.coli derived recombinant Human Oncostatin M.
    Top Product
    Discover our top product OSM Primary Antibody
  • 应用备注
    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhuman Oncostatin M (10 ng/mL), a concentration of 0.1 - 0.175 μg/mL of this antibody isrequired. Immunohistochemistry: We recommend use of 1 g per ml as a starting dilution. Paraffinsections may require pretreatment like Proteinase K or Trypsin predigestion to improvestaining. ELISA: To detect human Oncostatin M by direct ELISA (using 100 l/well antibody solution)a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant human Oncostatin M. Western Blot: use a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatiblesecondary reagents the detection limit for recombinant human Oncostatin M is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
    缓冲液
    PBS, pH 7.2 without preservatives
    储存液
    Without preservative
    注意事项
    Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
    储存条件
    4 °C/-20 °C
    储存方法
    Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
  • 抗原
    Oncostatin M (OSM)
    别名
    Oncostatin-M (OSM 产品)
    别名
    OSM antibody, OncoM antibody, oncostatin M antibody, OSM antibody, Osm antibody
    背景
    Synonyms: OSM
    分子量
    approx. 150 KDa
    基因ID
    5008
    NCBI登录号
    NP_065391
    UniProt
    P13725
    途径
    JAK/STAT Signaling, Negative Regulation of Hormone Secretion
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