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CCL18 抗体

CCL18 适用: 人 WB, EIA, Func 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN116179
发货至: 中国
  • 抗原 See all CCL18 抗体
    CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))
    适用
    • 26
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    宿主
    • 20
    • 6
    克隆类型
    • 21
    • 5
    多克隆
    标记
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    This CCL18 antibody is un-conjugated
    应用范围
    • 19
    • 9
    • 6
    • 5
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    • 2
    • 1
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    Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)
    特异性
    Specific for Human Macrophage Inflammatory Protein-4 (MIP-4).
    纯化方法
    Affinity Chromatography using an immobilized Human MIP-4 matrix
    免疫原
    Highly pure (>98%) E.coli derived recombinant Human MIP-4
    Top Product
    Discover our top product CCL18 Primary Antibody
  • 应用备注
    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhMIP-4 (100.0 ng/mL), a concentration of 8.0-10.0 μg/mL of this antibody is required. ELISA: (Indirect): To detect hMIP-4 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2 - 0.4 ng/well of recombinant hMIP-4. (Sandwich): To detect hMIP-4 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human MIP-4 (PP1054B) as a detectionantibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-4. Western Blot: To detect hMIP-4 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMIP-4 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
    限制
    仅限研究用
  • 溶解方式
    Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
    缓冲液
    PBS, pH 7.2 without preservatives
    储存液
    Without preservative
    注意事项
    Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • 抗原
    CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))
    别名
    MIP4 / CCL18 (CCL18 产品)
    背景
    This chemotactic factor that attracts lymphocytes but not monocytes or granulocytes may be involved in B cell migration into B cell follicles in lymph nodes. It attracts naive T lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T cells, CD4+ and CD8+ T cells and thus may play a role in both humoral and cell-mediated immunity responses. It is expressed at high levels in lung, lymph nodes, placenta, and bone marrow, not expressed by peripheral blood monocytes, and a monocyte-to-macrophage differentiation is a prerequisite for expression. Specifically induced in macrophages by IL4, IL13, and IL10, it belongs to the intercrine beta (chemokine CC) family.Synonyms: AMAC1, Alternative macrophage activation-associated CC chemokine 1, C-C motif chemokine 18, CC chemokine PARC, DCCK1, Dendritic cell chemokine 1, MIP-4, Macrophage inflammatory protein 4, PARC, Pulmonary and activation-regulated chemokine, SCYA18, Small-inducible cytokine A18
    基因ID
    6362
    NCBI登录号
    NP_002979
    UniProt
    P55774
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