CCL18 抗体
Quick Overview for CCL18 抗体 (ABIN116179)
抗原
See all CCL18 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- Specific for Human Macrophage Inflammatory Protein-4 (MIP-4).
-
纯化方法
- Affinity Chromatography using an immobilized Human MIP-4 matrix
-
免疫原
- Highly pure (>98%) E.coli derived recombinant Human MIP-4
-
-
anti-Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18) (AA 1-89) antibody
CCL18 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18) (AA 21-89) antibodyCCL18 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 2C6 unconjugated
anti-Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18) (C-Term) antibodyCCL18 适用: 人, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18) (Middle Region) antibodyCCL18 适用: 人, 犬, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18) antibodyCCL18 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18) (AA 21-89) antibodyCCL18 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
-
-
应用备注
- Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhMIP-4 (100.0 ng/mL), a concentration of 8.0-10.0 μg/mL of this antibody is required. ELISA: (Indirect): To detect hMIP-4 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2 - 0.4 ng/well of recombinant hMIP-4. (Sandwich): To detect hMIP-4 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human MIP-4 (PP1054B) as a detectionantibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-4. Western Blot: To detect hMIP-4 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMIP-4 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
-
限制
- 仅限研究用
-
-
-
溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
-
缓冲液
- PBS, pH 7.2 without preservatives
-
储存液
- Without preservative
-
注意事项
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
-
储存条件
- 4 °C/-20 °C
-
储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
-
-
- CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))
-
别名
- MIP4 / CCL18
-
背景
- This chemotactic factor that attracts lymphocytes but not monocytes or granulocytes may be involved in B cell migration into B cell follicles in lymph nodes. It attracts naive T lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T cells, CD4+ and CD8+ T cells and thus may play a role in both humoral and cell-mediated immunity responses. It is expressed at high levels in lung, lymph nodes, placenta, and bone marrow, not expressed by peripheral blood monocytes, and a monocyte-to-macrophage differentiation is a prerequisite for expression. Specifically induced in macrophages by IL4, IL13, and IL10, it belongs to the intercrine beta (chemokine CC) family.Synonyms: AMAC1, Alternative macrophage activation-associated CC chemokine 1, C-C motif chemokine 18, CC chemokine PARC, DCCK1, Dendritic cell chemokine 1, MIP-4, Macrophage inflammatory protein 4, PARC, Pulmonary and activation-regulated chemokine, SCYA18, Small-inducible cytokine A18
-
基因ID
- 6362
-
NCBI登录号
- NP_002979
-
UniProt
- P55774
抗原
-
