CCL7 抗体
Quick Overview for CCL7 抗体 (ABIN116147)
抗原
See all CCL7 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody recognizes Human Macrophage Chemotactic Protein-3 (MCP-3/CCL7). Other species not tested.
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纯化方法
- Affinity chromatography.
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免疫原
- Highly pure (>98%) recombinant hMCP?3 (human Macrophage/Monocyte chemotactic protein-3).
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anti-Chemokine (C-C Motif) Ligand 7 (CCL7) (AA 30-79) antibody
CCL7 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 7 (CCL7) (AA 24-99) antibodyCCL7 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4B5 unconjugated
anti-Chemokine (C-C Motif) Ligand 7 (CCL7) (AA 24-99) antibodyCCL7 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 7 (CCL7) antibodyCCL7 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 7 (CCL7) (N-Term) antibodyCCL7 适用: 人, 马, Pig WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 7 (CCL7) (AA 24-97) antibodyCCL7 适用: 小鼠, 大鼠 ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 7 (CCL7) (AA 28-97) antibodyCCL7 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 7 (CCL7) (AA 24-99) antibodyCCL7 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 7 (CCL7) (AA 24-99) antibodyCCL7 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 7 (CCL7) (N-Term) antibodyCCL7 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhMCP-3 (100.0 ng/mL), a concentration of 3.0-4.0 μg/mL of this antibody is required. ELISA: (Indirect): To detect hMCP-3 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hMCP-3. (Sandwich): To detect hMCP-3 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human MCP-3 (PP1046B) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMCP-3. Western Blot: To detect hMCP-3 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMCP-3 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives
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储存液
- Without preservative
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注意事项
- Centrifuge vial prior to opening!
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储存条件
- 4 °C
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储存方法
- Store the antibody in the dark at 2-8 °C. Do Not Freeze! Avoid prolonged exposure to light.
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- CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))
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别名
- MCP3 / CCL7
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背景
- This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.Synonyms: C-C motif chemokine 7, CCL-7, MCP-3, Macrophage Chemotactic Protein-3, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, SCYA6, SCYA7, Small-inducible cytokine A7
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基因ID
- 6354
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NCBI登录号
- NP_006264
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UniProt
- P80098
抗原
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