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IL16 抗体

This anti-IL16 antibody is a 兔 多克隆 antibody detecting IL16 in WB, EIA 和 Func. Suitable for 人.
产品编号 ABIN116115
发货至: 中国

Quick Overview for IL16 抗体 (ABIN116115)

抗原

See all IL16 抗体
IL16 (Interleukin 16 (IL16))

适用

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宿主

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克隆类型

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多克隆

标记

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This IL16 antibody is un-conjugated

应用范围

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Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)
  • 特异性

    Reacts with Human Interleukin-16. Other species not tested.

    纯化方法

    Affinity chromatography.

    免疫原

    Highly pure (>98%) recombinant hIL-16 (human Interleukin-16).
  • 应用备注

    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhIL-16 (4.20 ng/mL), a concentration of 0.07-0.012 μg/mL of this antibody is required. ELISA: To detect hIL-16 by direct ELISA (using 100l/well antibody solution) a concentrationof at least 0.5g/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/wellof recombinant hIL-16. Western Blot: To detect hIL-16 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-16 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

    缓冲液

    PBS, pH 7.2 without preservatives

    储存液

    Without preservative

    注意事项

    Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • 抗原

    IL16 (Interleukin 16 (IL16))

    别名

    Interleukin-16 / IL16

    背景

    IL16 is a proinflammatory cytokine that is chemotactic for CD4+ T lymphocytes, monocytes, and eosinophils. IL16 is synthesized as a precursor molecule (pro IL16) of approximately 68 kDa and 631 amino acid residues lacking a signal peptide. The sequence and structure of IL16 is conserved across species. In addition to inducing chemotaxis, IL16 upregulates the IL2 receptor and upregulates HLADR expression. It also inhibits T cell receptor (TCR)/CD3 dependent activation, and suppresses HIV1 replication in vitro. Sources of IL16 include epithelial cells, mast cells, T lymphocytes (CD4+ and CD8+), macrophages, synovial fibroblasts, and eosinophils.Synonyms: IL-16, LCF, Lymphocyte chemoattractant factor

    基因ID

    3603

    NCBI登录号

    NP_001165599

    UniProt

    Q14005
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