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IL-5 抗体

IL5 适用: 人 WB, EIA, IHC (p), Func 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN116079
发货至: 中国
  • 抗原 See all IL-5 (IL5) 抗体
    IL-5 (IL5) (Interleukin 5 (IL5))
    适用
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    宿主
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    克隆类型
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    多克隆
    标记
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    This IL-5 antibody is un-conjugated
    应用范围
    • 62
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    Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)
    特异性
    This antibody reacts with Human Interleukin-5. Other species not tested.
    纯化方法
    Affinity Chromatography.
    免疫原
    Highly pure (>98%) recombinant Human IL-5.
    Top Product
    Discover our top product IL5 Primary Antibody
  • 应用备注
    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-5(0.45 ng/mL), a concentration of 0.05-0.09 μg/mL of this antibody is required. ELISA: To detect hIL-5 by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hIL-5. Western Blot: To detect hIL-5 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-5 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
    缓冲液
    PBS, pH 7.2 without preservatives.
    储存液
    Without preservative
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • 抗原
    IL-5 (IL5) (Interleukin 5 (IL5))
    别名
    Interleukin-5 / IL5 (IL5 产品)
    别名
    EDF antibody, IL-5 antibody, TRF antibody, Il-5 antibody, IL5 antibody, interleukin 5 antibody, IL5 antibody, Il5 antibody
    背景
    Interleukin 5 (IL5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL2, IL4 and growth hormone. Human and mouse IL5 have 70 % amino acid sequence homology.Synonyms: B-cell differentiation factor I, Eosinophil differentiation factor, IL-5, T-cell replacing factor, TRF
    基因ID
    3567
    NCBI登录号
    NP_000870
    UniProt
    P05113
    途径
    JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
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