IL-2 抗体
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北京 101111
Quick Overview for IL-2 抗体 (ABIN116067)
抗原
See all IL-2 (IL2) 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Affinity Chromatography.
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免疫原
- Highly purified (>98%) recombinant human Interleukin 2. Remarks: Mol. Weight: 150 kDa
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anti-Interleukin 2 (IL2) (AA 21-153) antibody
IL2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 (IL2) (AA 21-153) antibodyIL2 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C3 unconjugated
anti-Interleukin 2 (IL2) (AA 10-169) antibodyIL2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 (IL2) (AA 21-153) antibodyIL2 适用: 人 WB 宿主: 小鼠 Monoclonal C5 unconjugated
anti-Interleukin 2 (IL2) antibodyIL2 适用: 人 WB, ELISA, FACS 宿主: 兔 Monoclonal unconjugated
anti-Interleukin 2 (IL2) antibodyIL2 适用: 人 WB, ELISA, IP, ICC, FACS 宿主: 小鼠 Monoclonal 35C3 unconjugated
anti-Interleukin 2 (IL2) antibody (APC)IL2 适用: 人 ICFC 宿主: 小鼠 Monoclonal 35C3 APC
anti-Interleukin 2 (IL2) antibody (PE)IL2 适用: 人 FACS 宿主: 小鼠 Monoclonal 35C3 PE
anti-Interleukin 2 (IL2) (AA 21-155) antibodyIL2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 (IL2) (AA 10-169) antibodyIL2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C12 unconjugated
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应用备注
- NeutralisationTo yield one-half maximal inhibition [ND50] of the biological activity of Human IL-2 (2.0 ng/mL), a concentration of 0.12-0.16 μg/mL of this antibody is required. ELISAIndirect: To detect hIL-2 by indirect ELISA (using 100 μL/well antibody solution) a concentration of0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-2. Sandwich: To detect hIL-2 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human IL-2 (PP1024B1 or PP1024B2) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-2. Western blotTo detect hIL-2 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant Human IL-2 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
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限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- IL-2 (IL2) (Interleukin 2 (IL2))
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别名
- Interleukin-2 / IL2
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背景
- Human interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells.Synonyms: IL-2, T-cell growth factor, TCGF
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基因ID
- 9606
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UniProt
- P60568
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
抗原
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