IGF2 抗体
Quick Overview for IGF2 抗体 (ABIN116059)
抗原
See all IGF2 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Affinity chromatography employing immobilized hhIGF-II matrix
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免疫原
- Highly pure (>98%) recombinant human IGF-II.
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anti-Insulin-Like Growth Factor 2 (IGF2) (AA 25-180) antibody
IGF2 适用: 人 IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 8H1 unconjugated
anti-Insulin-Like Growth Factor 2 (IGF2) (AA 25-180) antibodyIGF2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 2 (IGF2) (AA 39-68) antibodyIGF2 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 2 (IGF2) (AA 39-68) antibodyIGF2 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB18750 unconjugated
anti-Insulin-Like Growth Factor 2 (IGF2) (AA 25-91) antibodyIGF2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 2 (IGF2) antibodyIGF2 适用: 人, 大鼠, 小鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 2 (IGF2) (AA 1-180) antibodyIGF2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 2 (IGF2) (AA 46-77), (Middle Region) antibodyIGF2 适用: 人 WB, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 2 (IGF2) (AA 25-51) antibodyIGF2 适用: 人, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 2 (IGF2) (AA 25-91) antibodyIGF2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- NeutralizationTo yield one-half maximal inhibition [ND50] of the biological activity of hIGF-II (10 ng/mL), aconcentration of 3.1 - 4.6 μg/mL of this antibody is required. ElisaIndirect: To detect hIGF-II by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-II. Sandwich: To detect hIGF-II by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with Biotinylated Anti-HumanIGF-II as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIGF-II. Western BlotTo detect hIGF-II by Western Blot analysis this antibody can be used at a concentration of0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant hIGF-II is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
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限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2
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注意事项
- This product is photosensitive and should be protected from light. Should it contain a precipitate we recommend microcentrifugation before use.
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
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- IGF2 (Insulin-Like Growth Factor 2 (IGF2))
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别名
- Insulin-Like Growth Factor II / IGF2
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背景
- The IGF2 gene encodes a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is expressed only from the paternally inherited allele and is an imprinted gene which is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3' region and to the upstream INS gene at the 5' region. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. Insulin Like Growth Factor-2 (IGF-2) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types.Synonyms: IGF-II, Somatomedin-A
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基因ID
- 3481
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NCBI登录号
- NP_000603
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UniProt
- P01344
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途径
- Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation
抗原
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