CX3CL1 抗体 (Biotin)
Quick Overview for CX3CL1 抗体 (Biotin) (ABIN116015)
抗原
See all CX3CL1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Human Fractalkine.
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纯化方法
- Affinity chromatography
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免疫原
- Highly pure (> 98%) recombinant hFractalkine (human Fractalkine)
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anti-Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 25-332) antibody
CX3CL1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 365-397) antibodyCX3CL1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 301-397) antibodyCX3CL1 适用: 人, 大鼠, 小鼠 WB, ELISA, ICC, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) antibodyCX3CL1 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 25-100) antibodyCX3CL1 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (Internal Region) antibodyVerified CX3CL1 适用: 人 IHC, ELISA, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 81-336) antibodyCX3CL1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (N-Term) antibodyCX3CL1 适用: 人, 大鼠, 小鼠, Cow, 犬, 豚鼠, 马, 兔, Saccharomyces cerevisiae, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 272-306) antibodyCX3CL1 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB56920 unconjugated
anti-Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 83-330) antibodyCX3CL1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: (Direct): To detect hFractalkine by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hFractalkine. (Sandwich): To detect hFractalkine by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylatedpolyclonal antibody, in conjunction with Polyclonal Anti-Human Fractalkine (PP1009P) as acapture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinanthFractalkine. Western Blot: To detect hFractalkine by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFractalkine is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2, without preservatives
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized antibody is stable at -20 °C for one year from despatch. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.
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有效期
- 12 months
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- CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))
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别名
- Fractalkine / CX3CL1
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背景
- CX3CL1 is a member of the delta chemokine subfamily that contains a unique CX3C cysteine motif near the N-terminal. Unlike other known chemokines, it is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. CX3CL1, a leukocyte chemoattractant, is expressed in various tissues including the brain, heart, lung, kidney, skeletal muscle, and testis. The expression is reported to be up-regulated in endothelial cells and microglia by inflammatory signals. CX3CR1, a specific receptor for fractalkine, mediates both leukocyte migration and adhesion.Synonyms: C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, FKN, NTT, Neurotactin, SCYD1, Small-inducible cytokine D1
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基因ID
- 6376
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NCBI登录号
- NP_002987
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UniProt
- P78423
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途径
- Synaptic Membrane
抗原
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