CXCL5 抗体 (Biotin)
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- 抗原 See all CXCL5 抗体
- CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CXCL5 antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- This antibody reacts with human Epithelial Neutrophil Activity Protein-78 (hENA-78)
- 纯化方法
- Affinity chromatography.
- 免疫原
- Highly pure (>98%) recombinant hENA-78 (human ENA-78).
- Top Product
- Discover our top product CXCL5 Primary Antibody
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- 应用备注
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ELISA: (Direct): To detect hENA-78 by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hENA-78. (Sandwich): To detect hENA-78 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with Polyclonal Anti-Human ENA-78 (PP1003P) as a captureantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hENA-78. Western Blot: To detect hENA-78 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hENA-78 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
- 缓冲液
- PBS, pH 7.2 without preservatives.
- 储存液
- Without preservative
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- 抗原
- CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))
- 别名
- CXCL5 / ENA78 (CXCL5 产品)
- 别名
- ENA-78 antibody, SCYB5 antibody, Cxcl6 antibody, CXCL5 antibody, GCP-2 antibody, GCP2 antibody, SCYB6 antibody, AMCF-II antibody, LIX antibody, Scyb5 antibody, Scyb6 antibody, C-X-C motif chemokine ligand 5 antibody, C-X-C motif chemokine ligand 6 antibody, chemokine (C-X-C motif) ligand 5 antibody, C-X-C motif chemokine 5 antibody, CXCL5 antibody, Cxcl6 antibody, LOC701946 antibody, LOC703222 antibody, Cxcl5 antibody
- 背景
- CXCL5 is a a 9.9kD inflammatory chemokine that belongs to the CXC chemokine family. CXCL5 is expressed by fibroblasts, is induced by bacterial lipopolysaccharides, and is chemotactic for neutrophils. This chemokine is produced concomitantly with Interleukin 8 (IL8) in response to stimulation with either Interleukin 1 (IL1) or Tumor Necrosis Factor alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation.Synonyms: C-X-C motif chemokine 5, Cytokine LIX, ENA-78, Epithelial-derived neutrophil-activating protein 78, Neutrophil-activating peptide ENA-78, SCYB5, Small-inducible cytokine B5
- 基因ID
- 6374
- NCBI登录号
- NP_002985
- UniProt
- P42830
- 途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity
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