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IL-4 抗体

IL4 适用: 大鼠 WB, Func, EIA 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN115957
发货至: 中国
  • 抗原 See all IL-4 (IL4) 抗体
    IL-4 (IL4) (Interleukin 4 (IL4))
    适用
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    大鼠
    宿主
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    克隆类型
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    多克隆
    标记
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    This IL-4 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Functional Studies (Func), Enzyme Immunoassay (EIA)
    特异性
    Reacts with Rat IL-4
    纯化方法
    Affinity chromatography.
    免疫原
    Highly pure (>98%) recombinant rIL-4 (rat Interleukin-4).
    Top Product
    Discover our top product IL4 Primary Antibody
  • 应用备注
    Neutralization. ELISA: To detect Rat IL-4 by direct ELISA (using 100l/well antibody solution) a concentration of atleast 0.5g/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/wellof recombinant Rat IL-4. Western Blot: To detect Rat IL-4 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant Rat IL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
    缓冲液
    PBS, pH 7.2 without preservatives.
    储存液
    Without preservative
    注意事项
    Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • 抗原
    IL-4 (IL4) (Interleukin 4 (IL4))
    别名
    Interleukin-4 / IL4 (IL4 产品)
    别名
    IL4 antibody, IL-4 antibody, BCGF-1 antibody, BCGF1 antibody, BSF-1 antibody, BSF1 antibody, Il-4 antibody, Il4e12 antibody, IL2 antibody, interleukin 4 antibody, IL4 antibody, Il4 antibody
    背景
    IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naive CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra
    基因ID
    287287
    NCBI登录号
    NP_958427
    UniProt
    P20096
    途径
    JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
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