CNTF 抗体
Quick Overview for CNTF 抗体 (ABIN115937)
抗原
See all CNTF 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody recognises Rat CNTF.
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纯化方法
- Affinity Chromatography.
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免疫原
- Highly purified recombinant CNTF.
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anti-Ciliary Neurotrophic Factor (CNTF) (C-Term) antibody
CNTF 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor (CNTF) (AA 1-200) antibodyCNTF 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor (CNTF) (AA 2-198) antibodyCNTF 适用: 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor (CNTF) (AA 2-200) antibodyCNTF 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor (CNTF) (AA 2-200) antibodyCNTF 适用: 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor (CNTF) (AA 21-150) antibodyCNTF 适用: 人, 大鼠, 兔 WB, ELISA, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor (CNTF) (AA 2-198) antibodyCNTF 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor (CNTF) (AA 2-200) antibodyCNTF 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor (CNTF) (AA 4-196) antibodyCNTF 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor (CNTF) (AA 1-200) antibodyCNTF 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Rat CNTF (0.15 ng/mL), a concentration of 0.002-0.005 μg/mL of this antibody is required. ELISAIndirect: To detect Rat CNTF by indirect ELISA (using 100 l/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant Rat CNTF. Sandwich: To detect Rat CNTF by sandwich ELISA (using 100 l/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Rat CNTF as a detection antibody, allowsthe detection of at least 0.2-0.4 ng/well of recombinant Rat CNTF. Western BlotTo detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- CNTF (Ciliary Neurotrophic Factor (CNTF))
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别名
- CNTF
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背景
- CNTF is a potent neural factor that was originally characterized as a vital factor for the survival of chick ciliary neurons in vitro. CNTF is also important for the survival of other neural cell types including primary sensory neurons, motor neurons, basal forebrain neurons and type 2 astrocytes. CNTF is highly conserved across species and exhibits cross-species bioactivity.Synonyms: Ciliary Neurotrophic Factor
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基因ID
- 25707
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NCBI登录号
- NP_037298
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UniProt
- P20294
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途径
- JAK/STAT Signaling
抗原
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