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CNTF 抗体 (Biotin)

This anti-CNTF antibody is a 兔 多克隆 antibody detecting CNTF in WB 和 EIA. Suitable for 大鼠.
产品编号 ABIN115935
发货至: 中国

Quick Overview for CNTF 抗体 (Biotin) (ABIN115935)

抗原

See all CNTF 抗体
CNTF (Ciliary Neurotrophic Factor (CNTF))

适用

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大鼠

宿主

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克隆类型

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多克隆

标记

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This CNTF antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), Enzyme Immunoassay (EIA)
  • 特异性

    This antibody recognises Rat CNTF.

    纯化方法

    Affinity Chromatography.

    免疫原

    Highly purified recombinant CNTF.
  • 应用备注

    ELISADirect: To detect Rat CNTF by indirect ELISA (using 100 ml/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant Rat CNTF. Sandwich: To detect Rat CNTF by sandwich ELISA (using 100 ml/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. The Biotinylated antibody inconjunction with Anti-Rat CNTF (PP030P1 or PP030P2) as a Capture antibody, allows thedetection of at least 0.2-0.4 ng/well of recombinant Rat CNTF. Western BlotTo detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

    缓冲液

    PBS, pH 7.2 without preservatives.

    储存液

    Without preservative

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • 抗原

    CNTF (Ciliary Neurotrophic Factor (CNTF))

    别名

    CNTF

    背景

    CNTF is a potent neural factor that was originally characterized as a vital factor for the survival of chick ciliary neurons in vitro. CNTF is also important for the survival of other neural cell types including primary sensory neurons, motor neurons, basal forebrain neurons and type 2 astrocytes. CNTF is highly conserved across species and exhibits cross-species bioactivity.Synonyms: Ciliary Neurotrophic Factor

    基因ID

    25707

    NCBI登录号

    NP_037298

    UniProt

    P20294

    途径

    JAK/STAT Signaling
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