Ccl12 抗体
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Quick Overview for Ccl12 抗体 (ABIN115901)
抗原
See all Ccl12 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody reacts with Macrophage Chemotactic Protein-5 (MCP-5)
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纯化方法
- Affinity chromatography.
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免疫原
- Highly pure (>98%) recombinant mMCP-5.
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anti-Chemokine (C-C Motif) Ligand 12 (Ccl12) antibody
Ccl12 适用: 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 12 (Ccl12) antibody (Biotin)Ccl12 适用: 小鼠 ELISA, WB, Neut 宿主: 兔 Polyclonal Biotin
anti-Chemokine (C-C Motif) Ligand 12 (Ccl12) (AA 28-104) antibodyCcl12 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mMCP-5 (100.00 ng/mL), a concentration of 2.0-3.0 μg/mL of this antibody is required. ELISA: To detect mMCP-5 by direct ELISA (using 100 l/well antibody solution) a concentration ofat least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/wellof recombinant mMCP-5. Western Blot: To detect mMCP-5 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mMCP-5 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))
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别名
- MCP-5 / CCL12
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背景
- MCP-5 is a member of the b-chemokine family (C-C) of cytokines. This chemokine is strongly chemotactic for human monocytes and murine peritoneal macrophages, but is not active on mouse neutrophils and shows only a weak response on mouse eosinophils at high concentrations.Synonyms: C-C motif chemokine 12. Macrophage Chemotactic Protein-5, CCL-12, MCP-1-related chemokine, MCP5, Monocyte chemoattractant protein 5, Monocyte chemotactic protein 5, Small-inducible cytokine A12
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基因ID
- 100504977
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NCBI登录号
- NP_035461
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UniProt
- Q62401
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途径
- Cellular Response to Molecule of Bacterial Origin
抗原
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