IL-4 抗体
Quick Overview for IL-4 抗体 (ABIN115881)
抗原
See all IL-4 (IL4) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognises Mouse Interleukin-4 (IL-4).
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纯化方法
- Affinity chromatography.
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免疫原
- Highly pure (>98%) recombinant mIL-4 (murine Interleukin-4).
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anti-Interleukin 4 (IL4) (AA 25-153) antibody
IL4 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C4 unconjugated
anti-Interleukin 4 (IL4) (AA 25-153) antibodyIL4 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) (AA 122-150) antibodyIL4 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) (AA 61-111) antibodyIL4 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Interleukin 4 (IL4) (AA 25-153) antibodyIL4 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) (Internal Region) antibodyIL4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) (AA 122-150), (AA 122-151), (C-Term) antibodyIL4 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB22885 unconjugated
anti-Interleukin 4 (IL4) antibodyIL4 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) (AA 26-136) antibodyIL4 适用: 小鸡 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) antibody (PE)IL4 适用: 人, 猕猴 FACS 宿主: 大鼠 Monoclonal MP4-25D2 PE
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应用备注
- Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mIL-4 (7.5 ng/mL), aconcentration of 0.25-0.40 μg/mL of this antibody is required. ELISA: (Indirect): To detect mIL-4 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant mIL-4. (Sandwich): To detect mIL-4 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Murine IL-4 (PP011B) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-4. Western Blot: To detect mIL-4 by Western Blot analysis this antibody can be used at a concentration of
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限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- IL-4 (IL4) (Interleukin 4 (IL4))
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别名
- Interleukin-4 / IL4
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背景
- IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naive CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra
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基因ID
- 16189
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NCBI登录号
- NP_067258
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UniProt
- P07750
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
抗原
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