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IL-2 抗体

IL2 适用: 小鼠 WB, EIA, Func 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN115873
发货至: 中国
  • 抗原 See all IL-2 (IL2) 抗体
    IL-2 (IL2) (Interleukin 2 (IL2))
    适用
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    小鼠
    宿主
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    克隆类型
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    多克隆
    标记
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    This IL-2 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)
    特异性
    Detects Murine IL-2
    纯化方法
    Affinity chromatography
    免疫原
    Highly pure (>98%) recombinant mIL-2
    Top Product
    Discover our top product IL2 Primary Antibody
  • 应用备注
    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mIL-2 (2.0 ng/mL), aconcentration of 0.04-0.05 μg/mL of this antibody is required. ELISA: (Indirect): To detect mIL-2 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant mIL-2. (Sandwich): To detect mIL-2 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Murine IL-2 (PP009B) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-2. Western Blot: To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mIL-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
    缓冲液
    PBS, pH 7.2 without preservatives
    储存液
    Without preservative
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody at 2-8 °C under subdued light for one month or (in aliquots) at -20 °C for longer. This product is phototsensitive and should be protected from light.
  • 抗原
    IL-2 (IL2) (Interleukin 2 (IL2))
    别名
    Interleukin-2 / IL2 (IL2 产品)
    别名
    IL2 antibody, IL-2 antibody, il2 antibody, TCGF antibody, lymphokine antibody, Il-2 antibody, POIL2 antibody, interleukin 2 antibody, interleukin-15 antibody, IL2 antibody, il2 antibody, LOC100303688 antibody, Il2 antibody
    背景
    Interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells.Synonyms: IL-2, T-cell growth factor, TCGF
    基因ID
    16183
    NCBI登录号
    NP_032392
    UniProt
    P04351
    途径
    JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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