ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of thisantibody will detect at least 1000 pg/mL of recombinant Human MIG when used withbiotinylated anti-Human MIG antibody (cat. PP1055B) as the detection antibody at aconcentration of at least 1 μg/mL. Western Blot: To detect Human MIG by Western Blot analysis this antibody can be used ata concentration of 0.50-2.0 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hMIG is 0.25-0.50 ng/lane, undernon-reducing conditions and 2.0-4.0 ng/lane, under reducing conditions. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhMIG (100 ng/mL), a concentration of 5.0-10.0 μg/mL of this antibody is required. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore in sterile water to a concentration of 1.0 mg/mL
缓冲液
PBS without preservatives
储存液
Without preservative
注意事项
Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
储存条件
4 °C/-20 °C
储存方法
Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
MIG (monokine induced by interferon-gamma), a member of the alpha-chemokine family (CXC) of cytokines, is produced by stimulated monocytes, macrophages and endothelial cells. It signals through the CXCR3 receptor. MIG selectively chemoattracts Th1 lymphocytes, and also exerts other activities including inhibition of tumor growth, angiogenesis, and inhibition of colony formation of hematopoietic progenitors. Human MIG is active on murine cells.Synonyms: C-X-C motif chemokine 9, CMK, CXCL9, Gamma-interferon-induced monokine, MIG, SCYB9, Small-inducible cytokine B9