CCL8 抗体
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北京 101111
Quick Overview for CCL8 抗体 (ABIN115779)
抗原
See all CCL8 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Reacts with Human Macrophage Chemotactic Protein-2 (MCP-2)
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纯化方法
- Affinity Chromatography on Protein A
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免疫原
- Highly pure (>98%) recombinant Human MCP-2
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亚型
- IgG1
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anti-Chemokine (C-C Motif) Ligand 8 (CCL8) (AA 24-99) antibody
CCL8 适用: 人 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 8 (CCL8) (AA 1-99) antibodyCCL8 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 8 (CCL8) (AA 20-94) antibodyCCL8 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 8 (CCL8) (AA 1-99) antibodyCCL8 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 8 (CCL8) (AA 24-99) antibodyCCL8 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 8 (CCL8) (C-Term) antibodyCCL8 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 8 (CCL8) (AA 23-99) antibodyCCL8 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 1.0-2.0 μg/mL of thisantibody will detect at least 100 pg/mL of recombinant human MCP-2 when used withBiotin anti-Human MCP-2 antibody (cat. PP1047B) as the detection antibody at aconcentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect Human MCP-2 by Western Blot analysis this antibody can be usedat a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hMCP-2 is 0.25-0.50 ng/lane, undernon-reducing conditions. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman MCP-2 (100 ng/mL), a concentration of 0.8-1.0 μg/mL of this antibody is required.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 1.0 mg/mL.
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缓冲液
- PBS without preservatives
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))
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别名
- MCP2 / CCL8
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背景
- MCP 2 (Monocyte Chemoattractant Protein 2) is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. MCP 2 is important to inflammatory host responses, and is found in the highest concentration in the small intestine and peripheral blood cells. This protein is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection.Synonyms: C-C motif chemokine 8, CCL-8, HC14, MCP-2, Monocyte Chemotactic Protein-2, Monocyte chemoattractant protein 2, SCYA10, SCYA8, Small-inducible cytokine A8
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基因ID
- 6355
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NCBI登录号
- NP_005614
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UniProt
- P80075
抗原
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