IL-2 抗体
Quick Overview for IL-2 抗体 (ABIN115762)
抗原
See all IL-2 (IL2) 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Affinity Chromatography on Protein A
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免疫原
- Highly purified (>98%) E.coli derived recombinant Human IL-2 (Cat.-No PA075)
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亚型
- IgG1
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anti-Interleukin 2 (IL2) (AA 21-153) antibody
IL2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 (IL2) (AA 21-153) antibodyIL2 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C3 unconjugated
anti-Interleukin 2 (IL2) (AA 10-169) antibodyIL2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 (IL2) (AA 21-153) antibodyIL2 适用: 人 WB 宿主: 小鼠 Monoclonal C5 unconjugated
anti-Interleukin 2 (IL2) antibodyIL2 适用: 人 WB, ELISA, IP, ICC, FACS 宿主: 小鼠 Monoclonal 35C3 unconjugated
anti-Interleukin 2 (IL2) antibody (APC)IL2 适用: 人 ICFC 宿主: 小鼠 Monoclonal 35C3 APC
anti-Interleukin 2 (IL2) antibody (PE)IL2 适用: 人 FACS 宿主: 小鼠 Monoclonal 35C3 PE
anti-Interleukin 2 (IL2) (AA 21-153) antibodyIL2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 (IL2) antibodyIL2 适用: 人 WB, ELISA, FACS 宿主: 兔 Monoclonal unconjugated
anti-Interleukin 2 (IL2) (AA 21-155) antibodyIL2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Sandwich ELISA: In a Sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant Human IL-2 whenused with Biotin conjugated anti-Human IL-2 (cat. PP1024B) as the detection antibody at aconcentration of approximately 1.0-2.0 μg/mL. Western Blot: To detect Human IL-2 by Western Blot analysis this antibody can be used at aconcentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Human IL-2 is 2.0-4.0 ng/lane, under reducing ornon-reducing conditions. Immunohistochemistry on Formalin-Fixed Paraffin-Embedded Sections: 2.5-5.0 μg/mL withan overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with aDAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer isrecommended. Positive Control: Human Breast invasive ductal carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 1.0 mg/mL.
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缓冲液
- PBS without preservatives
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- IL-2 (IL2) (Interleukin 2 (IL2))
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别名
- Interleukin-2 / IL2
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背景
- Interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells.Synonyms: IL-2, T-cell growth factor, TCGF
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基因ID
- 9606
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UniProt
- P60568
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
抗原
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