AGRP 抗体
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北京 101111
Quick Overview for AGRP 抗体 (ABIN115499)
抗原
See all AGRP 抗体适用
宿主
克隆类型
标记
应用范围
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序列
- MKHHHHHHHM LVPRGSAQMG LAPMEGIRRP DQALLPELPG LGLRAPLKKT TAEQAEEDLL QEAQALAEVL DLQDREPRSS RRCVRLHESC LGQQVPCCDP CATCYCRFFN AFCYCRKLGT AMNPCSRT
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特异性
- The antibody was raised in rabbits by immunization with the recombinant Agouti-Related Protein.
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纯化方法
- Immunoaffinity Chromatography.
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免疫原
- Recombinant human Agouti-related protein from E.coli.
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anti-Agouti Related Protein Homolog (Mouse) (AGRP) (AA 21-131) antibody
AGRP 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Agouti Related Protein Homolog (Mouse) (AGRP) (AA 20-130) antibodyAGRP 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Agouti Related Protein Homolog (Mouse) (AGRP) (AA 83-132) antibodyAGRP 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Agouti Related Protein Homolog (Mouse) (AGRP) (AA 82-131) antibodyAGRP 适用: 人, 小鼠, 大鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Agouti Related Protein Homolog (Mouse) (AGRP) (AA 34-127) antibodyAGRP 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Agouti Related Protein Homolog (Mouse) (AGRP) (C-Term) antibodyVerified AGRP 适用: 人 IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
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应用备注
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ELISA. Western Blot. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 0.05 mL of deionized water.
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浓度
- 1.0 mg/mL
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缓冲液
- 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store (in aliquots) at -20 °C.
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- AGRP (Agouti Related Protein Homolog (Mouse) (AGRP))
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别名
- Agouti-Related Protein (AGRP)
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背景
- Agouti-related protein is an endogenous antagonist of hypothalamic alpha-melanocortin receptors MC3R and MC4R with potent orexigenic activity. Although a complete deletion of the AGRP gene does not produce any significant metabolic phenotypes, reduction in AGRP expression by RNA interference is associated with increased metabolic rate along with reduced weight gain. In hypothalamus, it is produced by neurons in the medial portion of arcuate nucleus, which produce also the potent orexigenic peptide Neuropeptide Y (NP-Y). Another site of central AGRP production is the hypothalamic nucleus. AGRP encompasses 132 amino acid residues and its alpha-melanocortin inhibiting activity results in a 34 amino acid cystine knot domain within the C-terminal (87-132) portion of the protein. Both AGRP and NP-Y expression was shown to be supressed by leptin. Central administration of AGRP induces hyperphagia and increased gain in body weight in rodents, but may also exert metabolic effects even when hyperphagia is prevented. In the absence of hyperphagia, intracerebralventricular administration of AGRP caused significant increases in plasma leptin and insulin concentrations (two-fold and 1.5-fold, respectively) and fat pad mass. In the perifery, AGRP mRNA was found in adrenal glands, lung, testis, ovary, skeletal muscle and adipose tissue in humans or rodents. In the adrenals, it was shown that AGRP antagonizes glucosteroid production mediated by MC4R. AGRP could then modulate locally the functions of some peripheral tissues such as adrenals. In human and rat serum, detectable levels of AGRP-like activity were reported in the lower picogram range. The serum AGRP levels were elevated in obese humans compared to lean controls and increased with fasting in rats. Total 128 AA. MW: 14.4 kDa (calculated). N-Terminal His-tag, 16 extra AA (highlighted).Synonyms: AGRT, ART
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基因ID
- 181
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NCBI登录号
- NP_001129
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UniProt
- O00253
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途径
- Feeding Behaviour, Photoperiodism
抗原
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