Immunoblotting (1: 100 - 1: 1000). Immunocytochemistry. Immunohistochemistry on frozen and paraffin-embedded tissues (1: 25- 1: 200 forimmunohistochemistry with avidin-biotinylated horseradish peroxidase complec (ABC) asdetection regant). Flow cytometry (1: 25-1: 200) Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
PBS, 0.09 % Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
抗原
KRT17
(Keratin 17 (KRT17))
别名
Cytokeratin 17
背景
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immuno-histochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.Synonyms: CK17, Cytokeratin-17, K17, KRT17, Keratin 17, Keratin type I cytoskeletal 17, Keratin-17