Reactive with natural and recombinant Human IL-16. This monoclonal antibody exhibits no detectable cross-reacting with Human Albumin Serum, Bovine Albumin Serum and other Human cytokines or growth factors tested such as IL-1beta, IL-8, IL-7 MCAF, TGF-beta1, EGF, bFGF, GM-CSF, M-CSF, TNF-alpha.
ELISA: This antibody can be used as a capture antibody in sandwich ELISA applications forhuman IL-16 detection in combination with a monoclonal tracer/detection antibody. Suggested Capture Coating Dose: 0.3g/well, Substrate: TMB. If the above suggested conditions are followed, approximately 20pg/mL of IL-16 inserum/plasma or 15 pg/mL of IL-16 in cell medium/urine can be detected with an assayrange of 0-1000 pg/mL Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with Double distillated water is recommended and to adjust the final concentration to 1.0 mg/mL
缓冲液
0.01 M PBS, pH 7.2
储存条件
4 °C
储存方法
Store the antibody at 2 - 8 °C. Do not freeze.
抗原
IL16
(Interleukin 16 (IL16))
别名
Interleukin-16 / IL16
背景
IL16 is a proinflammatory cytokine that is chemotactic for CD4+ T lymphocytes, monocytes, and eosinophils. IL16 is synthesized as a precursor molecule (pro IL16) of approximately 68 kDa and 631 amino acid residues lacking a signal peptide. The sequence and structure of IL16 is conserved across species. In addition to inducing chemotaxis, IL16 upregulates the IL2 receptor and upregulates HLADR expression. It also inhibits T cell receptor (TCR)/CD3 dependent activation, and suppresses HIV1 replication in vitro. Sources of IL16 include epithelial cells, mast cells, T lymphocytes (CD4+ and CD8+), macrophages, synovial fibroblasts, and eosinophils.Synonyms: IL-16, LCF, Lymphocyte chemoattractant factor