MHC Class I (RT1Ac) 抗体 (FITC)
Quick Overview for MHC Class I (RT1Ac) 抗体 (FITC) (ABIN114394)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G Chromatography.
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免疫原
- Phytohaemagglutinin Blasts.
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亚型
- IgG2a
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anti-MHC Class I (RT1Ac) antibody
适用: 大鼠 FACS, IHC (fro) 宿主: 小鼠 Monoclonal OX-27 unconjugated
anti-MHC Class I (RT1Ac) antibody (FITC)适用: 大鼠 FACS 宿主: 小鼠 Monoclonal OX-27 FITC
anti-MHC Class I (RT1Ac) antibody (Biotin)适用: 大鼠 FACS, IHC (fro) 宿主: 小鼠 Monoclonal OX-27 Biotin
anti-MHC Class I (RT1Ac) antibody适用: 大鼠 FACS, IHC (fro), IP 宿主: 小鼠 Monoclonal OX-27 unconjugated
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应用备注
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Flow cytometry: use 0.5 g of neat antibody to label 10^6 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Add. Information: Recognizes a polymorphic determinant of the MHC class I antigen in the rat. This Antibody can be used for labelling cells of donor or host origin in bone marrowchimeras. (1,2) -
实验流程
- FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell
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限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- PBS buffer with 0.02 % sodium azide as preservative and 0.5 % EIA grade BSA as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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- MHC Class I (RT1Ac)
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背景
- MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.Synonyms: MHC class I RT1.Ac heavy chain, RT1-A3
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基因ID
- 10116
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UniProt
- Q31255
抗原
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