MHC Class II I-Ad 抗体 (PE)
The PE-conjugated 小鼠 单克隆 anti-MHC Class II I-Ad antibody (Clone 34-5-3S) (ABIN114360) specifically detects MHC Class II I-Ad in FACS.
The antibody is reactive with 小鼠 samples.
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产品编号 ABIN114360
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for MHC Class II I-Ad 抗体 (PE) (ABIN114360)
抗原
MHC Class II I-Ad
适用
小鼠
宿主
小鼠
克隆类型
单克隆
标记
PE
应用范围
Flow Cytometry (FACS)
克隆位点
34-5-3S
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纯化方法
- Protein G affinity chromatography
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免疫原
- BDF splenocytes Remarks: R-Phycoerythrin conjugates are produced under license and protected under Stanford University held patents 4,520,110, 4,542,104, 4,859,582, 5,055,556 (U.S.): 76695 (EPC): 548440 (Australia): 1,179,942 (Canada): and 1,594,827 (Japan).
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亚型
- IgG2a
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应用备注
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Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 μl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0. 2 - 0. 1 μg of ABIN114360 per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most fluorochromes arelight sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 μl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 μl ofepropidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA.
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限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- PBS with 0.02 % sodium azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.
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有效期
- 6 months
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- MHC Class II I-Ad
抗原
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